PMID- 25807497
OWN - NLM
STAT- MEDLINE
DCOM- 20160303
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 3
DP  - 2015
TI  - Genome based phylogeny and comparative genomic analysis of intra-mammary 
      pathogenic Escherichia coli.
PG  - e0119799
LID - 10.1371/journal.pone.0119799 [doi]
LID - e0119799
AB  - Escherichia coli is an important cause of bovine mastitis and can cause both 
      severe inflammation with a short-term transient infection, as well as less 
      severe, but more chronic inflammation and infection persistence. E. coli is a 
      highly diverse organism that has been classified into a number of different 
      pathotypes or pathovars, and mammary pathogenic E. coli (MPEC) has been proposed 
      as a new such pathotype. The purpose of this study was to use genome sequence 
      data derived from both transient and persistent MPEC isolates (two isolates of 
      each phenotype) to construct a genome-based phylogeny that places MPEC in its 
      phylogenetic context with other E. coli pathovars. A subsidiary goal was to 
      conduct comparative genomic analyses of these MPEC isolates with other E. coli 
      pathovars to provide a preliminary perspective on loci that might be correlated 
      with the MPEC phenotype. Both concatenated and consensus tree phylogenies did not 
      support MPEC monophyly or the monophyly of either transient or persistent 
      phenotypes. Three of the MPEC isolates (ECA-727, ECC-Z, and ECA-O157) originated 
      from within the predominately commensal clade of E. coli, referred to as 
      phylogroup A. The fourth MPEC isolate, of the persistent phenotype (ECC-1470), 
      was sister group to an isolate of ETEC, falling within the E. coli B1 clade. This 
      suggests that the MPEC phenotype has arisen on numerous independent occasions and 
      that this has often, although not invariably, occurred from commensal ancestry. 
      Examination of the genes present in the MPEC strains relative to the commensal 
      strains identified a consistent presence of the type VI secretion system (T6SS) 
      in the MPEC strains, with only occasional representation in commensal strains, 
      suggesting that T6SS may be associated with MPEC pathogenesis and/or as an 
      inter-bacterial competitive attribute and therefore could represent a useful 
      target to explore for the development of MPEC specific inhibitors.
FAU - Richards, Vincent P
AU  - Richards VP
AD  - Department of Population Medicine and Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, New York, United States of America.
FAU - Lefebure, Tristan
AU  - Lefebure T
AD  - Department of Population Medicine and Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, New York, United States of America.
FAU - Pavinski Bitar, Paulina D
AU  - Pavinski Bitar PD
AD  - Department of Population Medicine and Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, New York, United States of America.
FAU - Dogan, Belgin
AU  - Dogan B
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Cornell 
      University, Ithaca, New York, United States of America.
FAU - Simpson, Kenneth W
AU  - Simpson KW
AD  - Department of Clinical Sciences, College of Veterinary Medicine, Cornell 
      University, Ithaca, New York, United States of America.
FAU - Schukken, Ynte H
AU  - Schukken YH
AD  - Department of Population Medicine and Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, New York, United States of America.
FAU - Stanhope, Michael J
AU  - Stanhope MJ
AD  - Department of Population Medicine and Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, New York, United States of America.
LA  - eng
SI  - GENBANK/AHHK00000000
SI  - GENBANK/AHHN00000000
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150325
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
EIN - PLoS One. 2015;10(7):e0133222. PMID: 26171614
MH  - Animals
MH  - Cattle
MH  - Escherichia coli/*genetics/isolation & purification
MH  - Escherichia coli Infections/*microbiology
MH  - Female
MH  - Genetic Variation
MH  - *Genome, Bacterial
MH  - Mastitis, Bovine/*microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sequence Analysis, DNA
PMC - PMC4373696
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/03/26 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/03/25
CRDT- 2015/03/26 06:00
PHST- 2014/10/08 00:00 [received]
PHST- 2015/01/22 00:00 [accepted]
PHST- 2015/03/26 06:00 [entrez]
PHST- 2015/03/26 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/03/25 00:00 [pmc-release]
AID - PONE-D-14-45210 [pii]
AID - 10.1371/journal.pone.0119799 [doi]
PST - epublish
SO  - PLoS One. 2015 Mar 25;10(3):e0119799. doi: 10.1371/journal.pone.0119799. 
      eCollection 2015.