PMID- 25807795
OWN - NLM
STAT- MEDLINE
DCOM- 20150925
LR  - 20220317
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 46
IP  - 1
DP  - 2015 Jan
TI  - [Tsc1 promoter methylation rate, mTOR expression in food-induced obese rat 
      hypothalamus].
PG  - 47-50
AB  - OBJECTIVE: To investigate the methylation rate of tuberous sclerosis complex 1 
      (Tsc1) promoter and expression of mammalian target of rapamycin (mTOR) in 
      food-induced rat hypothalamus. METHODS: 16 male SD rats were divided into high 
      fat diet induced group (8 rats) and normal control group (8 rats) feeding for 12 
      weeks. Body mass, mass of celiac fat, celiac fat/body mass were measured. 
      Methylation of Tsc1 promoter, mRNA and protein expression of mTOR were detected 
      by bisulfite sequencing method, RT-PCR and Western blot, respectively. RESULTS: 
      Mass of celiac fat, celiac fat/body mass were higher in food-induced rat than 
      that in control group. There were 11 methylation sites in SD rat hypothalamus. 
      Obese group has significantly higher methylation rates (94.50% +/- 4.66%) than 
      that of control group (86.60% +/- 3.49%) (P<0.002). The mRNA and protein 
      expression of mTOR were noted lower in control group than in obese group 
      (P<0.05). CONCLUSION: The increased methylation rate of Tsc1 promoter in 
      food-induced rat hypothalamus and up-regulated expression of mTOR, downstream 
      gene of Tsc1 may promote the obesity.
FAU - Zhang, Lin
AU  - Zhang L
FAU - Li, Ke
AU  - Li K
FAU - He, Hong
AU  - He H
FAU - Hu, Mao-qing
AU  - Hu MQ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Dietary Fats)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tsc1 protein, rat)
RN  - 0 (Tuberous Sclerosis Complex 1 Protein)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *DNA Methylation
MH  - Diet, High-Fat
MH  - Dietary Fats
MH  - Disease Models, Animal
MH  - Hypothalamus/*metabolism
MH  - Male
MH  - Obesity/*metabolism
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Tuberous Sclerosis Complex 1 Protein
MH  - Tumor Suppressor Proteins/*genetics
MH  - Up-Regulation
EDAT- 2015/03/27 06:00
MHDA- 2015/09/26 06:00
CRDT- 2015/03/27 06:00
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2015/09/26 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2015 Jan;46(1):47-50.