PMID- 25809504
OWN - NLM
STAT- MEDLINE
DCOM- 20161007
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 104
IP  - 2
DP  - 2016 Feb
TI  - Matrigel coatings for Parylene sheath neural probes.
PG  - 357-68
LID - 10.1002/jbm.b.33390 [doi]
AB  - The biologically derived hydrogel Matrigel (MG) was used to coat a Parylene-based 
      sheath intracortical electrode to act as a mechanical and biological buffer as 
      well as a matrix for delivering bioactive molecules to modulate the cellular 
      response and improve recording quality. MG was loaded with dexamethasone to 
      reduce the immune response together with nerve growth factor (NGF) and 
      brain-derived neurotrophic factor (BDNF) to maintain neuronal density and 
      encourage neuronal ingrowth toward electrodes within the sheath. Coating the 
      Parylene sheath electrode with the loaded MG significantly improved the 
      signal-to-noise ratio for neural events recorded from the motor cortex in rat for 
      more than 3 months. Electron microscopy showed even coverage of both the Parylene 
      substrate and the platinum recording electrodes. Electrochemical impedance 
      spectroscopy (EIS) of coated electrodes in 1x phosphate-buffered saline 
      demonstrated low impedance required for recording neural signals. This result was 
      confirmed by in vivo EIS data, showing significantly decreased impedance during 
      the first week of recording. Dexamethasone, NGF, and BDNF loaded into MG were 
      released within 1 day in 1x phosphate-buffered saline. Although previous studies 
      showed that MG loaded with either the immunosuppressant or the neurotrophic 
      factor cocktail provided modest improvement in recording quality in a 1-month in 
      vivo study, the combination of these bioactive molecules did not improve the 
      signal quality over coating probes with only MG in a 3-month in vivo study. The 
      MG coating may further improve recording quality by optimizing the in vivo 
      release profile for the bioactive molecules.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Lee, Curtis D
AU  - Lee CD
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, California, 90089-1111.
FAU - Hara, Seth A
AU  - Hara SA
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, California, 90089-1111.
FAU - Yu, Lawrence
AU  - Yu L
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, California, 90089-1111.
FAU - Kuo, Jonathan T W
AU  - Kuo JT
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, California, 90089-1111.
FAU - Kim, Brian J
AU  - Kim BJ
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, California, 90089-1111.
FAU - Hoang, Tuan
AU  - Hoang T
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, California, 90089-1111.
FAU - Pikov, Victor
AU  - Pikov V
AD  - Neural Engineering Program, Huntington Medical Research Institutes, Pasadena, 
      California, 91105-3104.
FAU - Meng, Ellis
AU  - Meng E
AD  - Department of Biomedical Engineering, University of Southern California, Los 
      Angeles, California, 90089-1111.
AD  - Ming Hsieh Department of Electrical Engineering, University of Southern 
      California, Los Angeles, California, 90089-2560.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150323
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Polymers)
RN  - 0 (Proteoglycans)
RN  - 0 (Xylenes)
RN  - 119978-18-6 (matrigel)
RN  - 25722-33-2 (parylene)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9007-34-5 (Collagen)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cells, Cultured
MH  - Coated Materials, Biocompatible/*chemistry
MH  - Collagen/*chemistry
MH  - Dexamethasone/pharmacology
MH  - Drug Combinations
MH  - *Electrodes, Implanted
MH  - Laminin/*chemistry
MH  - Male
MH  - Motor Cortex/cytology/*metabolism
MH  - Motor Neurons/cytology/*metabolism
MH  - Nerve Growth Factor/pharmacology
MH  - Polymers/*chemistry
MH  - Proteoglycans/*chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Xylenes/*chemistry
OTO - NOTNLM
OT  - Matrigel
OT  - Parylene
OT  - dexamethasone
OT  - intracortical electrode
OT  - neurotrophic factors
OT  - platinum
EDAT- 2015/03/27 06:00
MHDA- 2016/10/08 06:00
CRDT- 2015/03/27 06:00
PHST- 2014/09/08 00:00 [received]
PHST- 2014/12/26 00:00 [revised]
PHST- 2015/01/29 00:00 [accepted]
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
AID - 10.1002/jbm.b.33390 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2016 Feb;104(2):357-68. doi: 
      10.1002/jbm.b.33390. Epub 2015 Mar 23.