PMID- 25809662
OWN - NLM
STAT- MEDLINE
DCOM- 20160525
LR  - 20181202
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 103
IP  - 10
DP  - 2015 Oct
TI  - Coculture of dorsal root ganglion neurons and differentiated human corneal 
      stromal stem cells on silk-based scaffolds.
PG  - 3339-48
LID - 10.1002/jbm.a.35465 [doi]
AB  - Corneal tissue displays the highest peripheral nerve density in the human body. 
      Engineering of biomaterials to promote interactions between neurons and corneal 
      tissue could provide tissue models for nerve/cornea development, platforms for 
      drug screening, as well as innovative opportunities to regenerate cornea tissue. 
      The focus of this study was to develop a coculture system for differentiated 
      human corneal stromal stem cells (dhCSSCs) and dorsal root ganglion neurons (DRG) 
      to mimic the human cornea tissue interactions. Axon extension, connectivity, and 
      neuron cell viability were studied. DRG neurons developed longer axons when 
      cocultured with dhCSSCs in comparison to neuron cultures alone. To assess the 
      mechanism involved in the coculture response, nerve growth factors (NGF) secreted 
      by dhCSSCs including NGF, brain-derived neurotrophic factor (BDNF), glial 
      cell-derived neurotrophic factor (GDNF), and neurotrophin-3 were characterized 
      with greater focus on BDNF secretion. DhCSSCs also secreted collagen type I, an 
      extracellular matrix molecule favorable for neuronal outgrowth. This coculture 
      system provides a slowly degrading silk matrix to study neuronal responses in 
      concert with hCSSCs related to innervation of corneal tissue with utility toward 
      human corneal nerve regeneration and associated diseases.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Wang, Siran
AU  - Wang S
AD  - Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, 
      Massachusetts, 02155.
FAU - Ghezzi, Chiara E
AU  - Ghezzi CE
AD  - Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, 
      Massachusetts, 02155.
FAU - White, James D
AU  - White JD
AD  - Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, 
      Massachusetts, 02155.
FAU - Kaplan, David L
AU  - Kaplan DL
AD  - Department of Biomedical Engineering, Tufts University, 4 Colby St., Medford, 
      Massachusetts, 02155.
LA  - eng
GR  - R01 EY016415/EY/NEI NIH HHS/United States
GR  - R01 EY020856/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150525
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Silk)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Chick Embryo
MH  - Coculture Techniques
MH  - Ganglia, Spinal/cytology/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Neurons/cytology/*metabolism
MH  - Silk/*chemistry
MH  - Tissue Scaffolds/*chemistry
PMC - PMC4552610
MID - NIHMS678400
OTO - NOTNLM
OT  - coculture
OT  - collagen
OT  - cornea
OT  - neurons
OT  - silk
EDAT- 2015/03/27 06:00
MHDA- 2016/05/26 06:00
PMCR- 2016/10/01
CRDT- 2015/03/27 06:00
PHST- 2014/12/21 00:00 [received]
PHST- 2015/03/13 00:00 [revised]
PHST- 2015/03/23 00:00 [accepted]
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2016/05/26 06:00 [medline]
PHST- 2016/10/01 00:00 [pmc-release]
AID - 10.1002/jbm.a.35465 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2015 Oct;103(10):3339-48. doi: 10.1002/jbm.a.35465. Epub 
      2015 May 25.