PMID- 25810015
OWN - NLM
STAT- MEDLINE
DCOM- 20151214
LR  - 20181113
IS  - 1476-5586 (Electronic)
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Linking)
VI  - 17
IP  - 3
DP  - 2015 Mar
TI  - Characterizing the prevalence of chromosome instability in interval colorectal 
      cancer.
PG  - 306-16
LID - S1476-5586(15)00019-6 [pii]
LID - 10.1016/j.neo.2015.02.001 [doi]
AB  - A substantial proportion of colorectal cancers (CRCs) are interval CRCs (I-CRCs; 
      i.e., CRCs diagnosed soon after a colonoscopy). Chromosomal instability (CIN) is 
      defined as an increase in the rate of which whole chromosomes/large chromosomal 
      fragments are gained or lost and is observed in 85% of non-hereditary CRCs. The 
      contribution of CIN to the etiology of I-CRCs remains unknown. We established a 
      fluorescence in situ hybridization (FISH) approach to characterize CIN by 
      enumerating specific chromosomes and determined the prevalence of numerical CIN 
      in a population-based cohort of I-CRCs and control (sporadic) CRCs. Using the 
      population-based Manitoba Health administrative databases and Manitoba Cancer 
      Registry, we identified an age, sex, and colonic site of CRC matched cohort of 
      I-CRCs and controls and retrieved their archived paraffin-embedded tumor samples. 
      FISH chromosome enumeration probes specifically recognizing the pericentric 
      regions of chromosomes 8, 11, and 17 were first used on cell lines and then CRC 
      tissue microarrays to detect aneusomy, which was then used to calculate a CIN 
      score (CS). The 15th percentile CS for control CRC was used to define CIN 
      phenotype. Mean CSs were similar in the control CRCs and I-CRCs; 82% of I-CRCs 
      exhibited a CIN phenotype, which was similar to that in the control CRCs. This 
      study suggests that CIN is the most prevalent contributor to genomic instability 
      in I-CRCs. Further studies should evaluate CIN and microsatellite instability 
      (MSI) in the same cohort of I-CRCs to corroborate our findings and to further 
      assess concomitant contribution of CIN and MSI to I-CRCs.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Cisyk, A L
AU  - Cisyk AL
AD  - University of Manitoba, Winnipeg, Canada; Department of Biochemistry and Medical 
      Genetics, Winnipeg, Canada; Research Institute of Oncology and Hematology, 
      Winnipeg, Canada.
FAU - Penner-Goeke, S
AU  - Penner-Goeke S
AD  - University of Manitoba, Winnipeg, Canada; Department of Biochemistry and Medical 
      Genetics, Winnipeg, Canada; Research Institute of Oncology and Hematology, 
      Winnipeg, Canada.
FAU - Lichtensztejn, Z
AU  - Lichtensztejn Z
AD  - University of Manitoba, Winnipeg, Canada; Department of Biochemistry and Medical 
      Genetics, Winnipeg, Canada; Research Institute of Oncology and Hematology, 
      Winnipeg, Canada.
FAU - Nugent, Z
AU  - Nugent Z
AD  - CancerCare Manitoba, Winnipeg, Canada.
FAU - Wightman, R H
AU  - Wightman RH
AD  - University of Manitoba, Winnipeg, Canada; Department of Pathology, Winnipeg, 
      Canada; Grace Hospital, Winnipeg, Canada.
FAU - Singh, H
AU  - Singh H
AD  - University of Manitoba, Winnipeg, Canada; Department of Internal Medicine, Health 
      Sciences Centre, Winnipeg, Canada.
FAU - McManus, K J
AU  - McManus KJ
AD  - University of Manitoba, Winnipeg, Canada; Department of Biochemistry and Medical 
      Genetics, Winnipeg, Canada; Research Institute of Oncology and Hematology, 
      Winnipeg, Canada. Electronic address: Kirk.McManus@med.umanitoba.ca.
LA  - eng
GR  - MOP 115179/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
SB  - IM
MH  - Age Factors
MH  - Aged
MH  - Cell Line, Tumor
MH  - *Chromosomal Instability
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 8
MH  - Colorectal Neoplasms/*diagnosis/epidemiology/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Male
MH  - Microsatellite Instability
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Staging
MH  - Prevalence
MH  - Sensitivity and Specificity
MH  - Sex Factors
PMC - PMC4372653
EDAT- 2015/03/27 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/03/23
CRDT- 2015/03/27 06:00
PHST- 2014/11/26 00:00 [received]
PHST- 2015/01/29 00:00 [revised]
PHST- 2015/02/04 00:00 [accepted]
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/03/23 00:00 [pmc-release]
AID - S1476-5586(15)00019-6 [pii]
AID - 10.1016/j.neo.2015.02.001 [doi]
PST - ppublish
SO  - Neoplasia. 2015 Mar;17(3):306-16. doi: 10.1016/j.neo.2015.02.001.