PMID- 25810689
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150326
LR  - 20220316
IS  - 1357-714X (Print)
IS  - 1369-1643 (Electronic)
IS  - 1357-714X (Linking)
VI  - 2015
DP  - 2015
TI  - Fluorescence In Situ Hybridization for MDM2 Amplification as a Routine Ancillary 
      Diagnostic Tool for Suspected Well-Differentiated and Dedifferentiated 
      Liposarcomas: Experience at a Tertiary Center.
PG  - 812089
LID - 10.1155/2015/812089 [doi]
LID - 812089
AB  - Background. The assessment of MDM2 gene amplification by fluorescence in situ 
      hybridization (FISH) has become a routine ancillary tool for diagnosing atypical 
      lipomatous tumor (ALT)/well-differentiated liposarcoma and dedifferentiated 
      liposarcoma (WDL/DDL) in specialist sarcoma units. We describe our experience of 
      its utility at our tertiary institute. Methods. All routine histology samples in 
      which MDM2 amplification was assessed with FISH over a 2-year period were 
      included, and FISH results were correlated with clinical and histologic findings. 
      Results. 365 samples from 347 patients had FISH for MDM2 gene amplification. 170 
      were positive (i.e., showed MDM2 gene amplification), 192 were negative, and 3 
      were technically unsatisfactory. There were 122 histologically benign cases 
      showing a histology:FISH concordance rate of 92.6%, 142 WDL/DDL (concordance 
      96.5%), and 34 cases histologically equivocal for WDL (concordance 50%). Of 64 
      spindle cell/pleomorphic neoplasms (in which DDL was a differential diagnosis), 
      21.9% showed MDM2 amplification. Of the cases with discrepant histology and FISH, 
      all but 3 had diagnoses amended following FISH results. For discrepancies of 
      benign histology but positive FISH, lesions were on average larger, more 
      frequently in "classical" (intra-abdominal or inguinal) sites for WDL/DDL and 
      more frequently core biopsies. Discrepancies of malignant histology but negative 
      FISH were smaller, less frequently in "classical" sites but again more frequently 
      core biopsies. Conclusions. FISH has a high correlation rate with histology for 
      cases with firm histologic diagnoses of lipoma or WDL/DDL. It is a useful 
      ancillary diagnostic tool in histologically equivocal cases, particularly in WDL 
      lacking significant histologic atypia or DDL without corresponding WDL component, 
      especially in larger tumors, those from intra-abdominal or inguinal sites or core 
      biopsies. There is a significant group of well-differentiated adipocytic 
      neoplasms which are difficult to diagnose on morphology alone, in which FISH for 
      MDM2 amplification is diagnostically contributory.
FAU - Thway, Khin
AU  - Thway K
AUID- ORCID: 0000-0001-9727-8030
AD  - Sarcoma Unit, Royal Marsden Hospital, London SW3 6JJ, UK.
FAU - Wang, Jayson
AU  - Wang J
AD  - Department of Histopathology, Royal Marsden Hospital, London SW3 6JJ, UK.
FAU - Swansbury, John
AU  - Swansbury J
AD  - Clinical Cytogenetics, Royal Marsden Hospital, Sutton, Surrey SM2 5NG, UK.
FAU - Min, Toon
AU  - Min T
AD  - Clinical Cytogenetics, Royal Marsden Hospital, Sutton, Surrey SM2 5NG, UK.
FAU - Fisher, Cyril
AU  - Fisher C
AUID- ORCID: 0000-0003-4315-7836
AD  - Sarcoma Unit, Royal Marsden Hospital, London SW3 6JJ, UK.
LA  - eng
PT  - Journal Article
DEP - 20150225
PL  - Egypt
TA  - Sarcoma
JT  - Sarcoma
JID - 9709257
PMC - PMC4355609
EDAT- 2015/03/27 06:00
MHDA- 2015/03/27 06:01
PMCR- 2015/02/25
CRDT- 2015/03/27 06:00
PHST- 2014/10/11 00:00 [received]
PHST- 2015/01/05 00:00 [revised]
PHST- 2015/01/19 00:00 [accepted]
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2015/03/27 06:01 [medline]
PHST- 2015/02/25 00:00 [pmc-release]
AID - 10.1155/2015/812089 [doi]
PST - ppublish
SO  - Sarcoma. 2015;2015:812089. doi: 10.1155/2015/812089. Epub 2015 Feb 25.