PMID- 25810725
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150326
LR  - 20200930
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2015
DP  - 2015
TI  - Neuroprotective and antiapoptotic activity of lineage-negative bone marrow cells 
      after intravitreal injection in a mouse model of acute retinal injury.
PG  - 620364
LID - 10.1155/2015/620364 [doi]
LID - 620364
AB  - We investigated effects of bone marrow-derived, lineage-negative cell (Lin(-)BMC) 
      transplantation in acute retinal injury. Lin(-)BMCs were intravitreally injected 
      into murine eyes at 24 h after NaIO3-induced injury. Morphology, function, and 
      expression of apoptosis-related genes, including brain-derived neurotrophic 
      factor (BDNF) and its receptor, were assessed in retinas at 7 days, 28 days, and 
      3 months after transplantation. Moreover, global gene expression at day 7 was 
      analyzed by RNA arrays. We observed that Lin(-)BMCs integrated into outer retinal 
      layers improving morphological retinal structure and induced molecular changes 
      such as downregulation of proapoptotic caspase-3 gene, a decrease in BAX/BCL-2 
      gene ratio, and significant elevation of BDNF expression. Furthermore, 
      transplanted Lin(-)BMCs differentiated locally into cells with a macrophage-like 
      phenotype. Finally, Lin(-)BMCs treatment was associated with generation of two 
      distinct transcriptomic patterns. The first relates to downregulated genes 
      associated with regulation of neuron cell death and apoptosis, response to 
      oxidative stress/hypoxia and external stimuli, and negative regulation of cell 
      proliferation. The second relates to upregulated genes associated with 
      neurological system processes and sensory perception. Collectively, our data 
      demonstrate that transplanted Lin(-)BMCs exert neuroprotective function against 
      acute retinal injury and this effect may be associated with their antiapoptotic 
      properties and ability to express neurotrophic factors.
FAU - Machalinska, Anna
AU  - Machalinska A
AD  - Department of Histology and Embryology, Pomeranian Medical University, Al. 
      Powstancow Wlkp. 72, 70-111 Szczecin, Poland ; Department of Ophthalmology, 
      Pomeranian Medical University, Al. Powstancow Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Roginska, Dorota
AU  - Roginska D
AUID- ORCID: 0000-0001-8884-837X
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Pius-Sadowska, Ewa
AU  - Pius-Sadowska E
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Kawa, Milosz P
AU  - Kawa MP
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Paczkowska, Edyta
AU  - Paczkowska E
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Rudnicki, Michal
AU  - Rudnicki M
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Lejkowska, Renata
AU  - Lejkowska R
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Baumert, Bartlomiej
AU  - Baumert B
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Wiszniewska, Barbara
AU  - Wiszniewska B
AD  - Department of Histology and Embryology, Pomeranian Medical University, Al. 
      Powstancow Wlkp. 72, 70-111 Szczecin, Poland.
FAU - Machalinski, Boguslaw
AU  - Machalinski B
AD  - Department of General Pathology, Pomeranian Medical University, Al. Powstancow 
      Wlkp. 72, 70-111 Szczecin, Poland.
LA  - eng
PT  - Journal Article
DEP - 20150224
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC4354968
EDAT- 2015/03/27 06:00
MHDA- 2015/03/27 06:01
PMCR- 2015/02/24
CRDT- 2015/03/27 06:00
PHST- 2014/10/15 00:00 [received]
PHST- 2015/01/02 00:00 [accepted]
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2015/03/27 06:01 [medline]
PHST- 2015/02/24 00:00 [pmc-release]
AID - 10.1155/2015/620364 [doi]
PST - ppublish
SO  - Stem Cells Int. 2015;2015:620364. doi: 10.1155/2015/620364. Epub 2015 Feb 24.