PMID- 25811944
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20221207
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Linking)
VI  - 86
IP  - 7
DP  - 2015 Jul
TI  - Expression of Leptin and Visfatin in Gingival Tissues of Chronic Periodontitis 
      With and Without Type 2 Diabetes Mellitus: A Study Using Enzyme-Linked 
      Immunosorbent Assay and Real-Time Polymerase Chain Reaction.
PG  - 882-9
LID - 10.1902/jop.2015.140434 [doi]
AB  - BACKGROUND: The aim of this study is to investigate the protein and gene 
      expression of leptin and visfatin in gingival tissue from patients with chronic 
      periodontitis (CP), patients with CP and type 2 diabetes mellitus (T2DM), and 
      healthy individuals. METHODS: The study includes 50 individuals: 10 healthy 
      individuals, 20 patients with CP, and 20 patients with CP and T2DM. Plaque index, 
      gingival index, probing depth, and clinical attachment loss were measured, and 
      gingival biopsies were obtained. Leptin and visfatin protein expression in 
      gingival tissues was determined using enzyme-linked immunosorbent assay, and 
      messenger RNA (mRNA) expression was measured via real-time polymerase chain 
      reaction. RESULTS: The highest leptin mRNA and protein expression was observed in 
      the control group and was significantly (P </=0.05) different from the CP and 
      CP+T2DM groups. Gingival tissues from patients with CP and T2DM had a significant 
      increase in visfatin and a decrease in leptin gene and protein expression (P 
      <0.05) compared with both controls and patients with CP. CONCLUSION: Expression 
      of leptin and visfatin in the gingival tissues suggests a possible role for these 
      adipokines in the pathogenesis of CP and T2DM.
FAU - Ghallab, Noha A
AU  - Ghallab NA
AD  - Department of Oral Medicine, Periodontology, and Diagnosis, Faculty of Oral and 
      Dental Medicine, Cairo University, Cairo, Egypt.
FAU - Amr, Eman M
AU  - Amr EM
AD  - Department of Oral Medicine, Periodontology, and Diagnosis, Faculty of Oral and 
      Dental Medicine, Cairo University, Cairo, Egypt.
FAU - Shaker, Olfat G
AU  - Shaker OG
AD  - Department of Medical Biochemistry, Faculty of Medicine, Cairo University.
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150326
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Blood Glucose)
RN  - 0 (Cytokines)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Leptin)
RN  - EC 2.4.2.12 (Nicotinamide Phosphoribosyltransferase)
RN  - EC 2.4.2.12 (nicotinamide phosphoribosyltransferase, human)
SB  - IM
MH  - Adult
MH  - Biopsy/methods
MH  - Blood Glucose/analysis
MH  - Chronic Periodontitis/complications/*metabolism
MH  - Cytokines/*analysis
MH  - Dental Plaque Index
MH  - Diabetes Mellitus, Type 2/blood/*complications
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gingiva/*chemistry
MH  - Glycated Hemoglobin/analysis
MH  - Humans
MH  - Leptin/*analysis
MH  - Male
MH  - Middle Aged
MH  - Nicotinamide Phosphoribosyltransferase/*analysis
MH  - Periodontal Attachment Loss/complications/metabolism
MH  - Periodontal Index
MH  - Periodontal Pocket/complications/metabolism
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Adipokines
OT  - diabetes mellitus
OT  - enzyme-linked immunosorbent assay
OT  - molecular biology
OT  - periodontitis
OT  - polymerase chain reaction
EDAT- 2015/03/27 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/03/27 06:00
PHST- 2015/03/27 06:00 [entrez]
PHST- 2015/03/27 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1902/jop.2015.140434 [doi]
PST - ppublish
SO  - J Periodontol. 2015 Jul;86(7):882-9. doi: 10.1902/jop.2015.140434. Epub 2015 Mar 
      26.