PMID- 25813711
OWN - NLM
STAT- MEDLINE
DCOM- 20160113
LR  - 20150423
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 295
DP  - 2015 Jun 4
TI  - Brain-derived neurotrophic factor inhibits osmotic swelling of rat retinal glial 
      (Muller) and bipolar cells by activation of basic fibroblast growth factor 
      signaling.
PG  - 175-86
LID - S0306-4522(15)00274-2 [pii]
LID - 10.1016/j.neuroscience.2015.03.037 [doi]
AB  - Water accumulation in retinal glial (Muller) and neuronal cells resulting in 
      cellular swelling contributes to the development of retinal edema and 
      neurodegeneration. Intravitreal administration of neurotrophins such as 
      brain-derived neurotrophic factor (BDNF) is known to promote survival of retinal 
      neurons. Here, we show that exogenous BDNF inhibits the osmotic swelling of 
      Muller cell somata induced by superfusion of rat retinal slices or freshly 
      isolated cells with a hypoosmotic solution containing barium ions. BDNF also 
      inhibited the osmotic swelling of bipolar cell somata in retinal slices, but 
      failed to inhibit the osmotic soma swelling of freshly isolated bipolar cells. 
      The inhibitory effect of BDNF on Muller cell swelling was mediated by activation 
      of tropomyosin-related kinase B (TrkB) and transactivation of fibroblast growth 
      factor receptors. Exogenous basic fibroblast growth factor (bFGF) fully inhibited 
      the osmotic swelling of Muller cell somata while it partially inhibited the 
      osmotic swelling of bipolar cell somata. Isolated Muller cells displayed 
      immunoreactivity of truncated TrkB, but not full-length TrkB. Isolated rod 
      bipolar cells displayed immunoreactivities of both TrkB isoforms. Data suggest 
      that the neuroprotective effect of exogenous BDNF in the retina is in part 
      mediated by prevention of the cytotoxic swelling of retinal glial and bipolar 
      cells. While BDNF directly acts on Muller cells by activation of TrkB, BDNF 
      indirectly acts on bipolar cells by inducing glial release of factors like bFGF 
      that inhibit bipolar cell swelling.
CI  - Copyright (c) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Berk, B-A
AU  - Berk BA
AD  - Institute of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 
      University of Leipzig, Leipzig, Germany; Paul Flechsig Institute of Brain 
      Research, Medical Faculty, University of Leipzig, Leipzig, Germany.
FAU - Vogler, S
AU  - Vogler S
AD  - Paul Flechsig Institute of Brain Research, Medical Faculty, University of 
      Leipzig, Leipzig, Germany.
FAU - Pannicke, T
AU  - Pannicke T
AD  - Paul Flechsig Institute of Brain Research, Medical Faculty, University of 
      Leipzig, Leipzig, Germany.
FAU - Kuhrt, H
AU  - Kuhrt H
AD  - Paul Flechsig Institute of Brain Research, Medical Faculty, University of 
      Leipzig, Leipzig, Germany.
FAU - Garcia, T B
AU  - Garcia TB
AD  - Paul Flechsig Institute of Brain Research, Medical Faculty, University of 
      Leipzig, Leipzig, Germany; Institute of Biological Sciences, Federal University 
      of Para, Belem, Brazil.
FAU - Wiedemann, P
AU  - Wiedemann P
AD  - Department of Ophthalmology and Eye Hospital, Medical Faculty, University of 
      Leipzig, Leipzig, Germany.
FAU - Reichenbach, A
AU  - Reichenbach A
AD  - Paul Flechsig Institute of Brain Research, Medical Faculty, University of 
      Leipzig, Leipzig, Germany.
FAU - Seeger, J
AU  - Seeger J
AD  - Institute of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 
      University of Leipzig, Leipzig, Germany.
FAU - Bringmann, A
AU  - Bringmann A
AD  - Department of Ophthalmology and Eye Hospital, Medical Faculty, University of 
      Leipzig, Leipzig, Germany. Electronic address: bria@medizin.uni-leipzig.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150324
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 24GP945V5T (Barium)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Barium/pharmacology
MH  - Brain-Derived Neurotrophic Factor/metabolism/*pharmacology
MH  - Edema/*drug therapy/etiology
MH  - Ependymoglial Cells/*drug effects
MH  - Female
MH  - Fibroblast Growth Factors/*metabolism
MH  - In Vitro Techniques
MH  - Male
MH  - Osmotic Pressure
MH  - Protein Kinase C/metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptor, trkB/metabolism
MH  - Retina/cytology
MH  - Retinal Bipolar Cells/*drug effects
MH  - Signal Transduction/*drug effects
MH  - Statistics, Nonparametric
MH  - Time Factors
OTO - NOTNLM
OT  - bipolar cell
OT  - brain-derived neurotrophic factor
OT  - cell swelling
OT  - glia
OT  - osmotic stress
OT  - retina
EDAT- 2015/03/31 06:00
MHDA- 2016/01/14 06:00
CRDT- 2015/03/28 06:00
PHST- 2014/09/22 00:00 [received]
PHST- 2015/03/17 00:00 [revised]
PHST- 2015/03/18 00:00 [accepted]
PHST- 2015/03/28 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/01/14 06:00 [medline]
AID - S0306-4522(15)00274-2 [pii]
AID - 10.1016/j.neuroscience.2015.03.037 [doi]
PST - ppublish
SO  - Neuroscience. 2015 Jun 4;295:175-86. doi: 10.1016/j.neuroscience.2015.03.037. 
      Epub 2015 Mar 24.