PMID- 25814933
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150327
LR  - 20200930
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 9
DP  - 2015
TI  - Prenatal stress is a vulnerability factor for altered morphology and biological 
      activity of microglia cells.
PG  - 82
LID - 10.3389/fncel.2015.00082 [doi]
LID - 82
AB  - Several lines of evidence suggest that the dysregulation of the immune system is 
      an important factor in the development of depression. Microglia are the resident 
      macrophages of the central nervous system and a key player in innate immunity of 
      the brain. We hypothesized that prenatal stress (an animal model of depression) 
      as a priming factor could affect microglial cells and might lead to 
      depressive-like disturbances in adult male rat offspring. We investigated the 
      behavioral changes (sucrose preference test, Porsolt test), the expression of C1q 
      and CD40 mRNA and the level of microglia (Iba1 positive) in 3-month-old control 
      and prenatally stressed male offspring rats. In addition, we characterized the 
      morphological and biochemical parameters of potentially harmful (NO, iNOS, IL-1beta, 
      IL-18, IL-6, TNF-alpha, CCL2, CXCL12, CCR2, CXCR4) and beneficial (insulin-like 
      growth factor-1 (IGF-1), brain derived neurotrophic factor (BDNF)) phenotypes in 
      cultures of microglia obtained from the cortices of 1-2 days old control and 
      prenatally stressed pups. The adult prenatally stressed rats showed behavioral 
      (anhedonic- and depression-like) disturbances, enhanced expression of microglial 
      activation markers and an increased number of Iba1-immunopositive cells in the 
      hippocampus and frontal cortex. The morphology of glia was altered in cultures 
      from prenatally stressed rats, as demonstrated by immunofluorescence microscopy. 
      Moreover, in these cultures, we observed enhanced expression of CD40 and MHC II 
      and release of pro-inflammatory cytokines, including IL-1beta, IL-18, TNF-alpha and 
      IL-6. Prenatal stress significantly up-regulated levels of the chemokines CCL2, 
      CXCL12 and altered expression of their receptors, CCR2 and CXCR4 while IGF-1 
      production was suppressed in cultures of microglia from prenatally stressed rats. 
      Our results suggest that prenatal stress may lead to excessive microglia 
      activation and contribute to the behavioral changes observed in depression in 
      adulthood.
FAU - Slusarczyk, Joanna
AU  - Slusarczyk J
AD  - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish 
      Academy of Sciences Krakow, Poland.
FAU - Trojan, Ewa
AU  - Trojan E
AD  - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish 
      Academy of Sciences Krakow, Poland.
FAU - Glombik, Katarzyna
AU  - Glombik K
AD  - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish 
      Academy of Sciences Krakow, Poland.
FAU - Budziszewska, Boguslawa
AU  - Budziszewska B
AD  - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish 
      Academy of Sciences Krakow, Poland.
FAU - Kubera, Marta
AU  - Kubera M
AD  - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish 
      Academy of Sciences Krakow, Poland.
FAU - Lason, Wladyslaw
AU  - Lason W
AD  - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish 
      Academy of Sciences Krakow, Poland.
FAU - Popiolek-Barczyk, Katarzyna
AU  - Popiolek-Barczyk K
AD  - Department of Pain Pharmacology, Institute of Pharmacology, Polish Academy of 
      Sciences Krakow, Poland.
FAU - Mika, Joanna
AU  - Mika J
AD  - Department of Pain Pharmacology, Institute of Pharmacology, Polish Academy of 
      Sciences Krakow, Poland.
FAU - Wedzony, Krzysztof
AU  - Wedzony K
AD  - Laboratory of Pharmacology and Brain Biostructure, Institute of Pharmacology, 
      Polish Academy of Sciences Krakow, Poland.
FAU - Basta-Kaim, Agnieszka
AU  - Basta-Kaim A
AD  - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish 
      Academy of Sciences Krakow, Poland.
LA  - eng
PT  - Journal Article
DEP - 20150312
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC4357262
OTO - NOTNLM
OT  - chemokines
OT  - cytokines
OT  - depressive-like behavior
OT  - inflammation
OT  - microglia
OT  - neurotrophic factors
OT  - prenatal stress
EDAT- 2015/03/31 06:00
MHDA- 2015/03/31 06:01
PMCR- 2015/01/01
CRDT- 2015/03/28 06:00
PHST- 2014/12/12 00:00 [received]
PHST- 2015/02/23 00:00 [accepted]
PHST- 2015/03/28 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/03/31 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2015.00082 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2015 Mar 12;9:82. doi: 10.3389/fncel.2015.00082. eCollection 
      2015.