PMID- 25814935
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150327
LR  - 20200930
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 9
DP  - 2015
TI  - Caloric restriction protects against electrical kindling of the amygdala by 
      inhibiting the mTOR signaling pathway.
PG  - 90
LID - 10.3389/fncel.2015.00090 [doi]
LID - 90
AB  - Caloric restriction (CR) has been shown to possess antiepileptic properties; 
      however its mechanism of action is poorly understood. CR might inhibit the 
      activity of the mammalian or mechanistic target of rapamycin (mTOR) signaling 
      cascade, which seems to participate crucially in the generation of epilepsy. 
      Thus, we investigated the effect of CR on the mTOR pathway and whether CR 
      modified epilepsy generation due to electrical amygdala kindling. The former was 
      studied by analyzing the phosphorylation of adenosine monophosphate-activated 
      protein kinase, protein kinase B and the ribosomal protein S6. The mTOR cascade 
      is regulated by energy and by insulin levels, both of which may be changed by CR; 
      thus we investigated if CR altered the levels of energy substrates in the blood 
      or the level of insulin in plasma. Finally, we studied if CR modified the 
      expression of genes that encode proteins participating in the mTOR pathway. CR 
      increased the after-discharge threshold and tended to reduce the after-discharge 
      duration, indicating an anti-convulsive action. CR diminished the phosphorylation 
      of protein kinase B and ribosomal protein S6, suggesting an inhibition of the 
      mTOR cascade. However, CR did not change glucose, beta-hydroxybutyrate or insulin 
      levels; thus the effects of CR were independent from them. Interestingly, CR also 
      did not modify the expression of any investigated gene. The results suggest that 
      the anti-epileptic effect of CR may be partly due to inhibition of the mTOR 
      pathway.
FAU - Phillips-Farfan, Bryan V
AU  - Phillips-Farfan BV
AD  - Laboratorio de Nutricion Experimental, Instituto Nacional de Pediatria Mexico 
      City, Mexico.
FAU - Rubio Osornio, Maria Del Carmen
AU  - Rubio Osornio Mdel C
AD  - Laboratorio de Neurofisiologia, Instituto Nacional de Neurologia y Neurocirugia 
      Mexico City, Mexico.
FAU - Custodio Ramirez, Veronica
AU  - Custodio Ramirez V
AD  - Laboratorio de Neurofisiologia, Instituto Nacional de Neurologia y Neurocirugia 
      Mexico City, Mexico.
FAU - Paz Tres, Carlos
AU  - Paz Tres C
AD  - Laboratorio de Neurofisiologia, Instituto Nacional de Neurologia y Neurocirugia 
      Mexico City, Mexico.
FAU - Carvajal Aguilera, Karla G
AU  - Carvajal Aguilera KG
AD  - Laboratorio de Nutricion Experimental, Instituto Nacional de Pediatria Mexico 
      City, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20150311
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC4356078
OTO - NOTNLM
OT  - caloric restriction
OT  - epilepsy
OT  - hippocampus
OT  - mTOR signaling cascade
OT  - neocortex
EDAT- 2015/03/31 06:00
MHDA- 2015/03/31 06:01
PMCR- 2015/01/01
CRDT- 2015/03/28 06:00
PHST- 2014/10/14 00:00 [received]
PHST- 2015/02/26 00:00 [accepted]
PHST- 2015/03/28 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/03/31 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2015.00090 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2015 Mar 11;9:90. doi: 10.3389/fncel.2015.00090. eCollection 
      2015.