PMID- 25816637
OWN - NLM
STAT- MEDLINE
DCOM- 20150722
LR  - 20191113
IS  - 0869-6047 (Print)
IS  - 0869-6047 (Linking)
IP  - 9-10
DP  - 2014
TI  - [Calf blood hemodializate reduces neurotoxic effect of hydrogen peroxide on human 
      neuroblastoma cells].
PG  - 10-4
AB  - OBJECTIVE: Our aim was to study the effect of calf blood gemodializat on 
      apoptosis and intracellular signaling pathways of neuroblastoma cells SK-N-SH 
      human. METHODS: Apoptosis was recorded by fluorescent microscopy using Hoechst 
      33342. Necrosis cells was monitored by propidium iodide. The fluorescence of the 
      cells was recorded on a fluorescence inverted microscope Keyence BZ8100 (Japan). 
      Formation of reactive oxygen species (ROS) in the cells of SK-N-SH was determined 
      using nitroblue tetrazolium by absorbance at 620 nm on a plate reader "Uniplan". 
      RESULTS: When adding hydrogen peroxide to the background of the calf blood 
      gemodializat been decreasing apoptosis of these cells with 43 to 17% relative to 
      apoptosis in the presence of a hydrogen peroxide. Under these conditions, the 
      calf blood gemodializat significantly reduced ROS formation in human 
      neuroblastoma cells SK-N-SH by the action of hydrogen peroxide. In these cells, 
      we investigated the influence of calf blood gemodializat on apoptosis and 
      intracellular signaling pathway involving mitogen-activated protein kinase 
      (p38MAPK), extracellular regulatory kinase (ERK), phosphatidylinositol 3-kinase 
      (PI-3K) and-Jun-N-terminal kinase (JNK) using their selective inhibitors. 
      CONCLUSION: It was shown that the mechanism of the protective effect of calf 
      blood gemodializat against peroxide-induced apoptosis in SK-N-SH dominant role is 
      played by p38 MAPK and PI-3K.
FAU - Yurinskaya, M M
AU  - Yurinskaya MM
FAU - Vinokurov, M G
AU  - Vinokurov MG
FAU - Astashkin, E I
AU  - Astashkin EI
FAU - Grachev, S V
AU  - Grachev SV
FAU - Orekhova, N S
AU  - Orekhova NS
FAU - Novikova, A N
AU  - Novikova AN
FAU - Sokolova, I N
AU  - Sokolova IN
LA  - rus
PT  - Journal Article
PL  - Russia (Federation)
TA  - Vestn Ross Akad Med Nauk
JT  - Vestnik Rossiiskoi akademii meditsinskikh nauk
JID - 9215641
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - OU13V1EYWQ (SB 203580)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cattle
MH  - Cell Line, Tumor
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/*toxicity
MH  - Imidazoles/pharmacology
MH  - MAP Kinase Kinase 4/antagonists & inhibitors/metabolism
MH  - Neuroblastoma/*drug therapy/metabolism/pathology
MH  - Neurotoxicity Syndromes/pathology
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Pyridines/pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - *Renal Dialysis
MH  - Signal Transduction/drug effects
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
EDAT- 2014/01/01 00:00
MHDA- 2015/07/23 06:00
CRDT- 2015/03/31 06:00
PHST- 2015/03/31 06:00 [entrez]
PHST- 2014/01/01 00:00 [pubmed]
PHST- 2015/07/23 06:00 [medline]
AID - 10.15690/vramn.v69i9-10.1125 [doi]
PST - ppublish
SO  - Vestn Ross Akad Med Nauk. 2014;(9-10):10-4. doi: 10.15690/vramn.v69i9-10.1125.