PMID- 25817234
OWN - NLM
STAT- MEDLINE
DCOM- 20150717
LR  - 20150513
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 130
DP  - 2015 Jun 1
TI  - Puerarin suppresses high glucose-induced MCP-1 expression via modulating histone 
      methylation in cultured endothelial cells.
PG  - 103-7
LID - S0024-3205(15)00150-2 [pii]
LID - 10.1016/j.lfs.2015.02.022 [doi]
AB  - AIMS: Hyperglycemia is commonly associated with microcirculation dysfunction. The 
      purpose of the present study was to investigate the epigenetic mechanism involved 
      in the repression of monocyte chemoattractant protein-1 (MCP-1) expression by 
      puerarin under high glucose (25mM) condition. MAIN METHODS: MCP-1 gene expression 
      was measured by Real-Time quantitative Polymerase Chain Reaction (RT-qPCR), the 
      histone 3 lysine 4 methylation (H3K4me) and lysine 9 methylation (H3K9me) were 
      evaluated using chromatin immunoprecipitation assay. KEY FINDINGS: Puerarin 
      significantly inhibited high glucose-induced upregulation of H3K4 di- and 
      tri-methylation (H3K4me2/3) on the MCP-1 gene promotor. Additionally, the 
      enrichment of H3K4 histone methyltransferases including MLL, menin and SET7 on 
      the MCP-1 promotor was increased, while the demethylase LSD1 was decreased in 
      EA.hy926 cells following exposure to high glucose. The changes of the above 
      enzymes were reversed by puerarin treatment. The mRNA expression of MCP-1 was 
      increased by LSD1 blockage, while was decreased by MLL3 blockage. SIGNIFICANCE: 
      Our findings suggested that puerarin plays a critical role in transcriptional 
      repression of high glucose-induced MCP-1 gene expression, at least in part due to 
      alteration of H3K4me2/3 methylation, thus possesses a therapeutic potential in 
      diabetes-induced vascular injuries.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Han, Peng
AU  - Han P
AD  - Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, 
      Xiamen 361003, China.
FAU - Gao, Dehong
AU  - Gao D
AD  - Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, 
      Xiamen 361003, China; Department of Pathology, The First Affiliated Hospital of 
      Xiamen University, Xiamen 361003, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, 
      Xiamen 361003, China.
FAU - Liu, Suhuan
AU  - Liu S
AD  - Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, 
      Xiamen 361003, China; Central Laboratory, The First Affiliated Hospital of Xiamen 
      University, Xiamen 361003, China.
FAU - Yang, Shuyu
AU  - Yang S
AD  - Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, 
      Xiamen 361003, China; Division of Endocrinology and Diabetes, The First 
      Affiliated Hospital of Xiamen University, Xiamen 361003, China. Electronic 
      address: xmyangshuyu@126.com.
FAU - Li, Xuejun
AU  - Li X
AD  - Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, 
      Xiamen 361003, China; Division of Endocrinology and Diabetes, The First 
      Affiliated Hospital of Xiamen University, Xiamen 361003, China. Electronic 
      address: xmlixuejun@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150325
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Chemokine CCL2)
RN  - 0 (Histones)
RN  - 0 (Isoflavones)
RN  - 0 (RNA, Messenger)
RN  - IY9XDZ35W2 (Glucose)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Chromatin Immunoprecipitation
MH  - Endothelial Cells/drug effects/metabolism
MH  - Epigenesis, Genetic
MH  - Glucose/*metabolism
MH  - Histones/*metabolism
MH  - Humans
MH  - Hyperglycemia/drug therapy/physiopathology
MH  - Isoflavones/*pharmacology
MH  - Methylation
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - Endothelial cell
OT  - Histone methylation
OT  - Monocyte chemoattractant protein-1
OT  - Puerarin
EDAT- 2015/03/31 06:00
MHDA- 2015/07/18 06:00
CRDT- 2015/03/31 06:00
PHST- 2014/09/29 00:00 [received]
PHST- 2015/01/10 00:00 [revised]
PHST- 2015/02/24 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/07/18 06:00 [medline]
AID - S0024-3205(15)00150-2 [pii]
AID - 10.1016/j.lfs.2015.02.022 [doi]
PST - ppublish
SO  - Life Sci. 2015 Jun 1;130:103-7. doi: 10.1016/j.lfs.2015.02.022. Epub 2015 Mar 25.