PMID- 25817711 OWN - NLM STAT- MEDLINE DCOM- 20150526 LR - 20240322 IS - 1479-6805 (Electronic) IS - 0022-0795 (Print) IS - 0022-0795 (Linking) VI - 225 IP - 1 DP - 2015 Apr TI - Type 2 diabetes alters bone and marrow blood flow and vascular control mechanisms in the ZDF rat. PG - 47-58 LID - 10.1530/JOE-14-0514 [doi] AB - Bone health and cardiovascular function are compromised in individuals with type 2 diabetes mellitus (T2DM). The purpose of this study was to determine whether skeletal vascular control mechanisms are altered during the progression of T2DM in Zucker diabetic fatty (ZDF) rats. Responses of the principal nutrient artery (PNA) of the femur from obese ZDF rats with prediabetes, short-term diabetes, and long-term diabetes to endothelium-dependent (acetylcholine) and -independent (sodium nitroprusside) vasodilation and potassium chloride, norepinephrine (NE), and a myogenic vasoconstrictor were determined in vitro. Few changes in the PNA vasomotor responses occurred for the prediabetic and short-term diabetic conditions. Endothelium-dependent and -independent vasodilation were reduced, and NE and myogenic vasoconstriction were increased in obese ZDF rats with long-term diabetes relative to lean age-matched controls. Differences in endothelium-dependent vasodilation of the femoral PNA between ZDF rats and controls were abolished by the nitric oxide synthase inhibitor N(G)-nitro-l-arginine methyl ester. The passive pressure-diameter response of the femoral PNA was also lower across a range of intraluminal pressures with long-term T2DM. Regional bone and marrow perfusion and vascular conductance, measured in vivo using radiolabeled microspheres, were lower in obese ZDF rats with long-term diabetes. These findings indicate that the profound impairment of the bone circulation may contribute to the osteopenia found to occur in long bones during chronic T2DM. CI - (c) 2015 Society for Endocrinology. FAU - Stabley, John N AU - Stabley JN AD - Department of Applied Physiology and KinesiologyUniversity of Florida, Gainesville, Florida 32611, USADepartment of Kinesiology and Applied PhysiologyUniversity of Delaware, Newark, Delaware 19713, USADepartment of KinesiologyKansas State University, Manhattan, Kansas 66506, USADepartment of NutritionFood and Exercise Science, College of Human Sciences, Florida State University, 242 Sandels Building, 120 Convocation Way, Tallahassee, Florida 32306, USA. FAU - Prisby, Rhonda D AU - Prisby RD AD - Department of Applied Physiology and KinesiologyUniversity of Florida, Gainesville, Florida 32611, USADepartment of Kinesiology and Applied PhysiologyUniversity of Delaware, Newark, Delaware 19713, USADepartment of KinesiologyKansas State University, Manhattan, Kansas 66506, USADepartment of NutritionFood and Exercise Science, College of Human Sciences, Florida State University, 242 Sandels Building, 120 Convocation Way, Tallahassee, Florida 32306, USA. FAU - Behnke, Bradley J AU - Behnke BJ AD - Department of Applied Physiology and KinesiologyUniversity of Florida, Gainesville, Florida 32611, USADepartment of Kinesiology and Applied PhysiologyUniversity of Delaware, Newark, Delaware 19713, USADepartment of KinesiologyKansas State University, Manhattan, Kansas 66506, USADepartment of NutritionFood and Exercise Science, College of Human Sciences, Florida State University, 242 Sandels Building, 120 Convocation Way, Tallahassee, Florida 32306, USA Department of Applied Physiology and KinesiologyUniversity of Florida, Gainesville, Florida 32611, USADepartment of Kinesiology and Applied PhysiologyUniversity of Delaware, Newark, Delaware 19713, USADepartment of KinesiologyKansas State University, Manhattan, Kansas 66506, USADepartment of NutritionFood and Exercise Science, College of Human Sciences, Florida State University, 242 Sandels Building, 120 Convocation Way, Tallahassee, Florida 32306, USA. FAU - Delp, Michael D AU - Delp MD AD - Department of Applied Physiology and KinesiologyUniversity of Florida, Gainesville, Florida 32611, USADepartment of Kinesiology and Applied PhysiologyUniversity of Delaware, Newark, Delaware 19713, USADepartment of KinesiologyKansas State University, Manhattan, Kansas 66506, USADepartment of NutritionFood and Exercise Science, College of Human Sciences, Florida State University, 242 Sandels Building, 120 Convocation Way, Tallahassee, Florida 32306, USA Department of Applied Physiology and KinesiologyUniversity of Florida, Gainesville, Florida 32611, USADepartment of Kinesiology and Applied PhysiologyUniversity of Delaware, Newark, Delaware 19713, USADepartment of KinesiologyKansas State University, Manhattan, Kansas 66506, USADepartment of NutritionFood and Exercise Science, College of Human Sciences, Florida State University, 242 Sandels Building, 120 Convocation Way, Tallahassee, Florida 32306, USA mdelp@fsu.edu. LA - eng GR - K01 AG031327/AG/NIA NIH HHS/United States GR - AG-31317/AG/NIA NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, U.S. Gov't, Non-P.H.S. PL - England TA - J Endocrinol JT - The Journal of endocrinology JID - 0375363 RN - 0 (Blood Glucose) SB - IM MH - Animals MH - Blood Flow Velocity/*physiology MH - Blood Glucose MH - Blood Pressure MH - Bone Marrow/*blood supply MH - Bone and Bones/*blood supply MH - Diabetes Mellitus, Type 2/*physiopathology MH - Obesity/metabolism MH - Rats MH - Rats, Zucker MH - Vasodilation/*drug effects/physiology PMC - PMC4379453 MID - NIHMS665518 OTO - NOTNLM OT - Zucker diabetic fatty rat OT - bone blood flow OT - diabetes OT - endothelium OT - vascular conductance COIS- Declaration of interest The authors state that they have no conflicts of interest. EDAT- 2015/03/31 06:00 MHDA- 2015/05/27 06:00 PMCR- 2015/08/01 CRDT- 2015/03/31 06:00 PHST- 2015/03/31 06:00 [entrez] PHST- 2015/03/31 06:00 [pubmed] PHST- 2015/05/27 06:00 [medline] PHST- 2015/08/01 00:00 [pmc-release] AID - 225/1/47 [pii] AID - 10.1530/JOE-14-0514 [doi] PST - ppublish SO - J Endocrinol. 2015 Apr;225(1):47-58. doi: 10.1530/JOE-14-0514.