PMID- 25818265
OWN - NLM
STAT- MEDLINE
DCOM- 20160329
LR  - 20161020
IS  - 1768-322X (Electronic)
IS  - 0248-4900 (Linking)
VI  - 107
IP  - 7
DP  - 2015 Jul
TI  - New cellular mechanisms of gap junction degradation and recycling.
PG  - 218-31
LID - 10.1111/boc.201400048 [doi]
AB  - BACKGROUND INFORMATION: Connexins (Cxs), the constitutive proteins of gap 
      junctions, are key actors of many physiological processes. Therefore, alterations 
      of Cx expression and degradation lead to the development of physiopathological 
      disorders. Because of the formation of a double membrane vesicle termed annular 
      gap junction (AGJ), gap junction degradation is a unique physiological process 
      for which many cellular aspects remain unclear. RESULTS: By using a combination 
      of time-lapse fluorescence microscopy and high-resolution transmission electron 
      microscopy, we evidenced new specific cellular events concerning gap junction 
      degradation and recycling. Indeed, by time lapse video microscopy we 
      demonstrated, for the first time to our knowledge, that an entire AGJ can be 
      fully recycled back to the plasma membrane. Moreover, we dissected the 
      degradative processes of gap junction by electron microscopy approaches. 
      Interestingly, in addition to canonical autophagy and heterophagy pathways, 
      previously described, we discovered that both pathways could sometimes 
      intermingle. Strikingly, our results also highlighted a new lysosome-based 
      autophagy pathway that could play a pivotal role in common autophagy degradation. 
      CONCLUSIONS: The present investigation reveals that AGJ degradation is a more 
      complex process that it was previously thought. First, a complete recycling of 
      the gap junction plaque after its internalisation could occur. Second, the 
      degradation of this peculiar double membrane structure is possible through 
      autophagy, heterophagy, hetero-autophagy or by lysosomal-based autophagy. 
      Altogether, this work underlines novel aspects of gap junction degradation that 
      could be extended to other cell biology processes.
CI  - (c) 2015 Societe Francaise des Microscopies and Societe de Biologie Cellulaire de 
      France. Published by John Wiley & Sons Ltd.
FAU - Carette, Diane
AU  - Carette D
AD  - INSERM U 1065, Team 5 <<Physiopathological control of germ cell proliferation: 
      genomic and non-genomic mechanisms>>, University of Nice Sophia-Antipolis, Nice 
      cedex 3, 06204, France.
AD  - UMR S1147, University Paris Descartes, Paris, 75006, France.
AD  - University of Versailles, Saint Quentin, 78035, France.
FAU - Gilleron, Jerome
AU  - Gilleron J
AD  - INSERM U 1065, Team 5 <<Physiopathological control of germ cell proliferation: 
      genomic and non-genomic mechanisms>>, University of Nice Sophia-Antipolis, Nice 
      cedex 3, 06204, France.
FAU - Denizot, Jean-Pierre
AU  - Denizot JP
AD  - Unite de Neurosciences, Information et Complexite, CNRS UPR3293, Gif-sur-Yvette, 
      91190, France.
FAU - Grant, Kirsty
AU  - Grant K
AD  - Unite de Neurosciences, Information et Complexite, CNRS UPR3293, Gif-sur-Yvette, 
      91190, France.
FAU - Pointis, Georges
AU  - Pointis G
AD  - INSERM U 1065, Team 5 <<Physiopathological control of germ cell proliferation: 
      genomic and non-genomic mechanisms>>, University of Nice Sophia-Antipolis, Nice 
      cedex 3, 06204, France.
FAU - Segretain, Dominique
AU  - Segretain D
AD  - UMR S1147, University Paris Descartes, Paris, 75006, France.
AD  - University of Versailles, Saint Quentin, 78035, France.
LA  - eng
PT  - Journal Article
DEP - 20150506
PL  - England
TA  - Biol Cell
JT  - Biology of the cell
JID - 8108529
RN  - 0 (Connexins)
SB  - IM
MH  - Autophagy/*physiology
MH  - Cell Membrane/genetics/*metabolism/ultrastructure
MH  - Connexins/genetics/*metabolism
MH  - Gap Junctions/genetics/*metabolism/ultrastructure
MH  - HeLa Cells
MH  - Humans
MH  - Lysosomes/genetics/*metabolism/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - *Proteolysis
OTO - NOTNLM
OT  - Autophagy
OT  - Connexin
OT  - Degradative processes
OT  - Endo-lysosomal pathway
OT  - Gap junction
EDAT- 2015/03/31 06:00
MHDA- 2016/03/30 06:00
CRDT- 2015/03/31 06:00
PHST- 2014/06/17 00:00 [received]
PHST- 2015/03/23 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/03/30 06:00 [medline]
AID - 10.1111/boc.201400048 [doi]
PST - ppublish
SO  - Biol Cell. 2015 Jul;107(7):218-31. doi: 10.1111/boc.201400048. Epub 2015 May 6.