PMID- 25818416
OWN - NLM
STAT- MEDLINE
DCOM- 20151214
LR  - 20220409
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 52
DP  - 2015 Jun
TI  - Development and functional evaluation of biomimetic silicone surfaces with 
      hierarchical micro/nano-topographical features demonstrates favourable in vitro 
      foreign body response of breast-derived fibroblasts.
PG  - 88-102
LID - S0142-9612(15)00113-1 [pii]
LID - 10.1016/j.biomaterials.2015.02.003 [doi]
AB  - Reproducing extracellular matrix topographical cues, such as those present within 
      acellular dermal matrix (ADM), in synthetic implant surfaces, may augment 
      cellular responses, independent of surface chemistry. This could lead to enhanced 
      implant integration and performance while reducing complications. In this work, 
      the hierarchical micro and nanoscale features of ADM were accurately and 
      reproducibly replicated in polydimethylsiloxane (PDMS), using an innovative 
      maskless 3D grayscale fabrication process not previously reported. Human breast 
      derived fibroblasts (n=5) were cultured on PDMS surfaces and compared to 
      commercially available smooth and textured silicone implant surfaces, for up to 
      one week. Cell attachment, proliferation and cytotoxicity, in addition to 
      immunofluorescence staining, SEM imaging, qRT-PCR and cytokine array were 
      performed. ADM PDMS surfaces promoted cell adhesion, proliferation and survival 
      (p=<0.05), in addition to increased focal contact formation and spread fibroblast 
      morphology when compared to commercially available implant surfaces. PCNA, 
      vinculin and collagen 1 were up-regulated in fibroblasts on biomimetic surfaces 
      while IL8, TNFalpha, TGFbeta1 and HSP60 were down-regulated (p=<0.05). A reduced 
      inflammatory cytokine response was also observed (p=<0.05). This study represents 
      a novel approach to the development of functionalised biomimetic prosthetic 
      implant surfaces which were demonstrated to significantly attenuate the acute in 
      vitro foreign body reaction to silicone.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Kyle, Daniel J T
AU  - Kyle DJ
AD  - Plastic and Reconstructive Surgery Research, Manchester Institute of 
      Biotechnology, University of Manchester, Manchester, UK; School of Computer 
      Science, Centre for Mesoscience and Nanotechnology, The University of Manchester, 
      Manchester, UK.
FAU - Oikonomou, Antonios
AU  - Oikonomou A
AD  - School of Computer Science, Centre for Mesoscience and Nanotechnology, The 
      University of Manchester, Manchester, UK.
FAU - Hill, Ernie
AU  - Hill E
AD  - School of Computer Science, Centre for Mesoscience and Nanotechnology, The 
      University of Manchester, Manchester, UK.
FAU - Bayat, Ardeshir
AU  - Bayat A
AD  - Plastic and Reconstructive Surgery Research, Manchester Institute of 
      Biotechnology, University of Manchester, Manchester, UK. Electronic address: 
      Ardeshir.Bayat@manchester.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150221
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Adult
MH  - Biomimetic Materials/*chemistry
MH  - Biomimetics/methods
MH  - Breast/*cytology/immunology
MH  - Breast Implants/*adverse effects
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Female
MH  - Fibroblasts/*cytology/immunology
MH  - Foreign-Body Reaction/*etiology/immunology
MH  - Humans
MH  - Inflammation/etiology/immunology
MH  - Middle Aged
MH  - Silicones/chemistry
MH  - Surface Properties
OTO - NOTNLM
OT  - Acellular dermal matrix (ADM)
OT  - Biomimetic topography
OT  - Capsular contracture
OT  - Cell-implant interface
OT  - Micro and nano patterning
OT  - Silicone breast implant
EDAT- 2015/03/31 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/03/31 06:00
PHST- 2014/07/31 00:00 [received]
PHST- 2015/01/25 00:00 [revised]
PHST- 2015/02/01 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - S0142-9612(15)00113-1 [pii]
AID - 10.1016/j.biomaterials.2015.02.003 [doi]
PST - ppublish
SO  - Biomaterials. 2015 Jun;52:88-102. doi: 10.1016/j.biomaterials.2015.02.003. Epub 
      2015 Feb 21.