PMID- 25818459
OWN - NLM
STAT- MEDLINE
DCOM- 20151231
LR  - 20150330
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 52
DP  - 2015 Jun
TI  - The utilization of decellularized tendon slices to provide an inductive 
      microenvironment for the proliferation and tenogenic differentiation of stem 
      cells.
PG  - 539-50
LID - S0142-9612(15)00185-4 [pii]
LID - 10.1016/j.biomaterials.2015.02.061 [doi]
AB  - The extracellular matrix (ECM) microenvironment for the stem cell niches, 
      including but not limited to the biochemical composition, matrix topography, and 
      stiffness, is crucial to stem cell proliferation and differentiation. The purpose 
      of this study was to explore the capacity of the decellularized tendon slices 
      (DTSs) to induce stem cell proliferation and tenogenic differentiation. Rat adult 
      stem cells, including tendon-derived stem cells (TDSCs) and bone marrow-derived 
      stem cells (BMSCs), were identified to have universal stem cell characteristics. 
      The DTSs were found to retain the native tendon ECM microenvironment cues, 
      including the inherent surface topography, well-preserved tendon ECM biochemical 
      composition and similar stiffness to native tendon. When the TDSCs and BMSCs were 
      cultured on the DTSs respectively, the LIVE/DEAD assay, alamarBlue(R) assay, 
      scanning electron microscopy examination and qRT-PCR analysis demonstrated that 
      the DTSs have the capacity to support these stem cells homogeneous distribution, 
      alignment, significant proliferation and tenogenic differentiation. Taken 
      together, the findings of this study indicate that the DTSs can provide a 
      naturally inductive microenvironment for the proliferation and tenogenic 
      differentiation of TDSCs and BMSCs, supporting the use of decellularized tendon 
      ECM as a promising and valuable approach for tendon repair/reconstruction.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Ning, Liang-Ju
AU  - Ning LJ
AD  - Institute of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, 
      Sichuan University, Chengdu, Sichuan 610041, China.
FAU - Zhang, Ya-Jing
AU  - Zhang YJ
AD  - Institute of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, 
      Sichuan University, Chengdu, Sichuan 610041, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Institute of Stem Cell and Tissue Engineering, Regenerative Medicine Research 
      Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
FAU - Qing, Quan
AU  - Qing Q
AD  - Institute of Stem Cell and Tissue Engineering, Regenerative Medicine Research 
      Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
FAU - Jiang, Yan-Lin
AU  - Jiang YL
AD  - Institute of Stem Cell and Tissue Engineering, Regenerative Medicine Research 
      Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
FAU - Yang, Jie-Liang
AU  - Yang JL
AD  - Institute of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, 
      Sichuan University, Chengdu, Sichuan 610041, China.
FAU - Luo, Jing-Cong
AU  - Luo JC
AD  - Institute of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, 
      Sichuan University, Chengdu, Sichuan 610041, China.
FAU - Qin, Ting-Wu
AU  - Qin TW
AD  - Institute of Stem Cell and Tissue Engineering, State Key Laboratory of 
      Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, 
      Sichuan University, Chengdu, Sichuan 610041, China. Electronic address: 
      tingwuqin@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150318
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Oxazines)
RN  - 0 (Xanthenes)
RN  - 1FN9YD6968 (resazurin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Extracellular Matrix/metabolism
MH  - Flow Cytometry
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Osteogenesis
MH  - Oxazines/chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/*cytology
MH  - Tendons/cytology/*pathology
MH  - Tissue Engineering
MH  - Xanthenes/chemistry
OTO - NOTNLM
OT  - Bone marrow-derived stem cells
OT  - Decellularized tendon slices
OT  - Differentiation
OT  - Microenvironment
OT  - Proliferation
OT  - Tendon-derived stem cells
EDAT- 2015/03/31 06:00
MHDA- 2016/01/01 06:00
CRDT- 2015/03/31 06:00
PHST- 2014/09/24 00:00 [received]
PHST- 2015/02/11 00:00 [revised]
PHST- 2015/02/13 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/01/01 06:00 [medline]
AID - S0142-9612(15)00185-4 [pii]
AID - 10.1016/j.biomaterials.2015.02.061 [doi]
PST - ppublish
SO  - Biomaterials. 2015 Jun;52:539-50. doi: 10.1016/j.biomaterials.2015.02.061. Epub 
      2015 Mar 18.