PMID- 25818978
OWN - NLM
STAT- MEDLINE
DCOM- 20150812
LR  - 20161125
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 196
IP  - 1
DP  - 2015 Jun 1
TI  - Hydrogen gas inhibits high-mobility group box 1 release in septic mice by 
      upregulation of heme oxygenase 1.
PG  - 136-48
LID - S0022-4804(15)00155-9 [pii]
LID - 10.1016/j.jss.2015.02.042 [doi]
AB  - BACKGROUND: Sepsis is a potentially fatal whole-body inflammation caused by 
      severe infection. Hydrogen gas (H2) is effective for treating sepsis. In this 
      study, we hypothesized that the protective function of H2 in mice with septic 
      lung injury occurred through the activation of heme oxygenase 1 (HO-1) and its 
      upstream regulator nuclear factor-erythroid 2 p45-related factor 2 (Nrf2). 
      MATERIALS AND METHODS: Male institute of cancer research mice were subjected to 
      sepsis by cecal ligation and puncture (CLP) with the presence or absence of H2. 
      Beginning at 1 and 6 h after CLP or sham operation, respectively, 2% H2 was 
      inhaled for 1 h. We intraperitoneally injected the HO-1 inhibitor zinc 
      protoporphyrin IX (40 mg/kg) 1 h before CLP. To assess the severity of septic 
      lung injury, we observed the 7-d survival rate, wet/dry weight ratio of lung, 
      lung histopathologic score, oxygenation index, and so forth. Serum and 
      homogenates from the lung, liver, and kidney were acquired for measuring the 
      levels of high-mobility group box 1 (HMGB1) at 6, 12, and 24 h after CLP or sham 
      operation. Furthermore, the protein and messenger RNA expression of Nrf2, HO-1, 
      and HMGB1 was measured at 6, 12, and 24 h. RESULTS: Septic mice had a lower 
      survival rate and more severe lung injury compared with the sham group. However, 
      therapy with H2 increased the survival rate and alleviated the severity of lung 
      injury, reduced the HMGB1 level, and increased the HO-1 and Nrf2 levels in septic 
      mice. Moreover, the HO-1 inhibitor zinc protoporphyrin IX significantly 
      eliminated the protective effect of H2 on septic lung injury. CONCLUSIONS: H2 
      plays a significant role in regulating the release of the inflammatory cytokine 
      HMGB1 in septic mice, which is partially mediated through the activation of HO-1 
      as a downstream molecule of Nrf2.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Anesthesiology, General Hospital of Tianjin Medical University, 
      Tianjin, China; Tianjin Institute of Anesthesiology, Tianjin, China.
FAU - Xie, Keliang
AU  - Xie K
AD  - Department of Anesthesiology, General Hospital of Tianjin Medical University, 
      Tianjin, China; Tianjin Institute of Anesthesiology, Tianjin, China. Electronic 
      address: xiekeliang2009@hotmail.com.
FAU - Chen, Hongguang
AU  - Chen H
AD  - Department of Anesthesiology, General Hospital of Tianjin Medical University, 
      Tianjin, China; Tianjin Institute of Anesthesiology, Tianjin, China.
FAU - Wang, Guolin
AU  - Wang G
AD  - Department of Anesthesiology, General Hospital of Tianjin Medical University, 
      Tianjin, China; Tianjin Institute of Anesthesiology, Tianjin, China.
FAU - Yu, Yonghao
AU  - Yu Y
AD  - Department of Anesthesiology, General Hospital of Tianjin Medical University, 
      Tianjin, China; Tianjin Institute of Anesthesiology, Tianjin, China. Electronic 
      address: yuyonghao@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150227
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (HMGB1 Protein)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Protoporphyrins)
RN  - 15442-64-5 (zinc protoporphyrin)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
SB  - IM
MH  - Animals
MH  - HMGB1 Protein/*antagonists & inhibitors
MH  - Heme Oxygenase-1/genetics/*physiology
MH  - Hydrogen/*pharmacology
MH  - Lung/pathology
MH  - Male
MH  - Membrane Proteins/genetics/*physiology
MH  - Mice
MH  - NF-E2-Related Factor 2/genetics
MH  - Peroxidase/metabolism
MH  - Protoporphyrins/pharmacology
MH  - Sepsis/*drug therapy/metabolism/pathology
MH  - Signal Transduction
MH  - Up-Regulation
OTO - NOTNLM
OT  - Acute lung injury
OT  - Heme oxygenase 1
OT  - High-mobility group box 1
OT  - Hydrogen gas
OT  - Sepsis
EDAT- 2015/03/31 06:00
MHDA- 2015/08/13 06:00
CRDT- 2015/03/31 06:00
PHST- 2014/11/22 00:00 [received]
PHST- 2015/02/15 00:00 [revised]
PHST- 2015/02/18 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/08/13 06:00 [medline]
AID - S0022-4804(15)00155-9 [pii]
AID - 10.1016/j.jss.2015.02.042 [doi]
PST - ppublish
SO  - J Surg Res. 2015 Jun 1;196(1):136-48. doi: 10.1016/j.jss.2015.02.042. Epub 2015 
      Feb 27.