PMID- 25819401
OWN - NLM
STAT- MEDLINE
DCOM- 20160412
LR  - 20181113
IS  - 1348-4400 (Electronic)
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 61
IP  - 3
DP  - 2015
TI  - Status of autophagy, lysosome activity and apoptosis during corpus luteum 
      regression in cattle.
PG  - 229-36
LID - 10.1262/jrd.2014-135 [doi]
AB  - Corpus luteum (CL) regression is required during the estrous cycle. During CL 
      regression, luteal cells stop producing progesterone and are degraded by 
      apoptosis. However, the detailed mechanism of CL regression in cattle has not 
      been fully elucidated. The aim of this study was to evaluate autophagy, lysosome 
      activity, and apoptosis during CL regression in cattle. The expression of 
      autophagy-related genes (LC3alpha, LC3beta, Atg3, and Atg7) and the protein LC3-II was 
      significantly higher in the late CL than in the mid CL. In addition, autophagy 
      activity was significantly increased in the late CL. Moreover, gene expression of 
      the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly 
      lower in the late CL than in the mid CL. Lysosome activation and expression of 
      cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in 
      the late CL and were associated with an increase in cathepsin B protein. In 
      addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, 
      were significantly higher in the late CL than in the mid CL. These results 
      suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes 
      and apoptotic mediators, which are involved in regression of the bovine CL.
FAU - Aboelenain, Mansour
AU  - Aboelenain M
AD  - Laboratory of Animal Breeding and Reproduction, Department of Animal Science, 
      Graduate School of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan.
FAU - Kawahara, Manabu
AU  - Kawahara M
FAU - Balboula, Ahmed Zaky
AU  - Balboula AZ
FAU - Montasser, Abd El-Monem
AU  - Montasser Ael-M
FAU - Zaabel, Samy Mowaed
AU  - Zaabel SM
FAU - Okuda, Kiyoshi
AU  - Okuda K
FAU - Takahashi, Masashi
AU  - Takahashi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150328
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 0 (RNA, Messenger)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 3.4.18.1 (Cathepsin Z)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.1 (Cathepsin B)
RN  - EC 3.4.23.5 (Cathepsin D)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - *Autophagy
MH  - Caspase 3/metabolism
MH  - Cathepsin B/metabolism
MH  - Cathepsin D/metabolism
MH  - Cathepsin Z/metabolism
MH  - Cattle
MH  - Corpus Luteum/*physiology
MH  - Estrous Cycle
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Luteal Cells/cytology
MH  - *Luteolysis
MH  - Lysosomes/*metabolism
MH  - Progesterone/metabolism
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC4498366
EDAT- 2015/03/31 06:00
MHDA- 2016/04/14 06:00
PMCR- 2015/06/01
CRDT- 2015/03/31 06:00
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - 2014-135 [pii]
AID - 10.1262/jrd.2014-135 [doi]
PST - ppublish
SO  - J Reprod Dev. 2015;61(3):229-36. doi: 10.1262/jrd.2014-135. Epub 2015 Mar 28.