PMID- 25820447
OWN - NLM
STAT- MEDLINE
DCOM- 20151013
LR  - 20150728
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 230
IP  - 11
DP  - 2015 Nov
TI  - Lactogenic hormone stimulation and epigenetic control of L-amino acid oxidase 
      expression in lactating mammary glands.
PG  - 2755-62
LID - 10.1002/jcp.25000 [doi]
AB  - L-amino acid oxidase (LAO), a classic flavoprotein, shows antibacterial activity 
      by producing hydrogen peroxide. LAO exists in many tissues such as salivary 
      gland, thymus, spleen, small intestine and testis. In particular, LAO was highly 
      expressed in mice milk and plays an important factor in innate immunity of 
      mammary glands. However, the mechanism which LAO expression is regulated 
      spatially and temporally in lactating mammary glands has been unclear. In this 
      study, we showed the contribution of lactogenic hormone and epigenetic control on 
      LAO gene expression. In monolayer of mammary epithelial cells, treatment of 
      lactogenic hormone mixture, dexamethasone, insulin and prolactin, did not induce 
      LAO mRNA expression and its promoter activity, even though one of milk protein 
      beta-casein expression was stimulated. However, increase of LAO expression was 
      observed when the cells were treated with lactogenic hormones in a 3-dimensional 
      culture. The results of chromatin immunoprecipitation analysis revealed that 
      histone H3K18 acetylation increased and histone H3K27 tri-methylation decreased 
      with lactation, which is associated with a period of high LAO expression. 
      Moreover, the treatment of histone methylation inhibitor (DZNep) as well as 
      histone deacetylation inhibitor (Trichostatine A) induced LAO expression in 
      monolayer of mammary cells. Taken together, this is the first demonstration 
      showing that LAO expression is induced in cell culture, and stimulation of 
      lactogenic hormone and change of histone modification are promising signals to 
      show highly expression of LAO in lactating mammary glands.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Fujii, Kazuki
AU  - Fujii K
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo, Japan.
FAU - Zhang, Haolin
AU  - Zhang H
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo, Japan.
FAU - Usuda, Kento
AU  - Usuda K
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo, Japan.
FAU - Watanabe, Gen
AU  - Watanabe G
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo, Japan.
FAU - Nagaoka, Kentaro
AU  - Nagaoka K
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo 
      University of Agriculture and Technology, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Histones)
RN  - 0 (Hydroxamic Acids)
RN  - 0 (RNA, Messenger)
RN  - 3X2S926L3Z (trichostatin A)
RN  - 9002-62-4 (Prolactin)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Animals
MH  - Epigenesis, Genetic
MH  - Female
MH  - Gene Expression Regulation
MH  - Histones/antagonists & inhibitors/metabolism
MH  - Hydroxamic Acids/administration & dosage
MH  - Immunity, Innate/genetics
MH  - L-Amino Acid Oxidase/*biosynthesis/metabolism
MH  - Lactation/*genetics
MH  - Mammary Glands, Animal/*metabolism
MH  - Methylation
MH  - Mice
MH  - Prolactin/*biosynthesis
MH  - RNA, Messenger/biosynthesis
EDAT- 2015/03/31 06:00
MHDA- 2015/10/16 06:00
CRDT- 2015/03/31 06:00
PHST- 2015/01/09 00:00 [received]
PHST- 2015/03/24 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - 10.1002/jcp.25000 [doi]
PST - ppublish
SO  - J Cell Physiol. 2015 Nov;230(11):2755-62. doi: 10.1002/jcp.25000.