PMID- 25820756
OWN - NLM
STAT- MEDLINE
DCOM- 20160420
LR  - 20181113
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 35
IP  - 6
DP  - 2015 Aug
TI  - Time Course of Behavioral Alteration and mRNA Levels of Neurotrophic Factor 
      Following Stress Exposure in Mouse.
PG  - 807-17
LID - 10.1007/s10571-015-0174-x [doi]
AB  - Stress is known to affect neurotrophic factor expression, which induces 
      depression-like behavior. However, whether there are time-dependent changes in 
      neurotrophic factor mRNA expression following stress remains unclear. In the 
      present study, we tested whether chronic stress exposure induces long-term 
      changes in depression-related behavior, serum corticosterone, and hippocampal 
      proliferation as well as neurotrophic factor family mRNA levels, such as 
      brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), 
      neurotrophin-3 (NT-3), and ciliary neurotrophic factor (CNTF), in the mouse 
      hippocampus. The mRNA level of neurotrophic factors (BDNF, NGF, NT-3, and CNTF) 
      was measured using the real-time PCR. The serum corticosterone level was 
      evaluated by enzyme-linked immunosorbent assay, and, for each subject, the 
      hippocampal proliferation was examined by 5-bromo-2-deoxyuridine immunostaining. 
      Mice exhibited depression-like behavior in the forced-swim test (FST) and 
      decreased BDNF mRNA and hippocampal proliferation in the middle of the stress 
      exposure. After 15 days of stress exposure, we observed increased immobility in 
      the FST, serum corticosterone levels, and BDNF mRNA levels and degenerated 
      hippocampal proliferation, maintained for at least 2 weeks. Anhedonia-like 
      behavior in the sucrose preference test and NGF mRNA levels were decreased 
      following 15 days of stress. NGF mRNA levels were significantly higher 1 week 
      after stress exposure. The current data demonstrate that chronic stress exposure 
      induces prolonged BDNF and NGF mRNA changes and increases corticosterone levels 
      and depression-like behavior in the FST, but does not alter other neurotrophic 
      factors or performance in the sucrose preference test.
FAU - Hashikawa, Naoya
AU  - Hashikawa N
AD  - Department of Life Science, Okayama University of Science, 1-1 Ridai-cho, 
      Kita-ku, Okayama, 700-0005, Japan.
FAU - Ogawa, Takumi
AU  - Ogawa T
FAU - Sakamoto, Yusuke
AU  - Sakamoto Y
FAU - Ogawa, Mami
AU  - Ogawa M
FAU - Matsuo, Yumi
AU  - Matsuo Y
FAU - Zamami, Yoshito
AU  - Zamami Y
FAU - Hashikawa-Hobara, Narumi
AU  - Hashikawa-Hobara N
LA  - eng
GR  - D1DA31340/DA/NIDA NIH HHS/United States
GR  - R01DA23979/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150328
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/*physiology
MH  - Cell Proliferation
MH  - Chronic Disease
MH  - Corticosterone/blood
MH  - Dentate Gyrus/pathology
MH  - Hippocampus/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Growth Factors/*genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - *Stress, Psychological/genetics/metabolism/pathology/physiopathology
MH  - Time Factors
EDAT- 2015/03/31 06:00
MHDA- 2016/04/21 06:00
CRDT- 2015/03/31 06:00
PHST- 2015/02/05 00:00 [received]
PHST- 2015/03/07 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/04/21 06:00 [medline]
AID - 10.1007/s10571-015-0174-x [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2015 Aug;35(6):807-17. doi: 10.1007/s10571-015-0174-x. Epub 
      2015 Mar 28.