PMID- 25820928
OWN - NLM
STAT- MEDLINE
DCOM- 20160829
LR  - 20151023
IS  - 1098-1063 (Electronic)
IS  - 1050-9631 (Linking)
VI  - 25
IP  - 11
DP  - 2015 Nov
TI  - Physical exercise and acute restraint stress differentially modulate hippocampal 
      brain-derived neurotrophic factor transcripts and epigenetic mechanisms in mice.
PG  - 1380-92
LID - 10.1002/hipo.22458 [doi]
AB  - Physical exercise and stressful experiences have been shown to exert opposite 
      effects on behavioral functions and brain plasticity, partly by involving the 
      action of brain-derived neurotrophic factor (BDNF). Although epigenetic 
      modifications are known to play a pivotal role in the regulation of the different 
      BDNF transcripts, it is poorly understood whether epigenetic mechanisms are also 
      implied in the BDNF modulation induced by physical exercise and stress. Here, we 
      show that total BDNF mRNA levels and BDNF transcripts 1, 2, 3, 4, 6, and 7 were 
      reduced immediately after acute restraint stress (RS) in the hippocampus of mice, 
      and returned to control levels 24 h after the stress session. On the contrary, 
      exercise increased BDNF mRNA expression and counteracted the stress-induced 
      decrease of BDNF transcripts. Physical exercise-induced up-regulation of BDNF 
      transcripts was accounted for by increase in histone H3 acetylated levels at 
      specific BDNF promoters, whereas the histone H3 trimethylated lysine 27 and 
      dimethylated lysine 9 levels were unaffected. Acute RS did not change the levels 
      of acetylated and methylated histone H3 at the BDNF promoters. Furthermore, we 
      found that physical exercise and RS were able to differentially modulate the 
      histone deacetylases mRNA levels. Finally, we report that a single treatment with 
      histone deacetylase inhibitors, prior to acute stress exposure, prevented the 
      down-regulation of total BDNF and BDNF transcripts 1, 2, 3, and 6, partially 
      reproducing the effect of physical exercise. Overall, these results suggest that 
      physical exercise and stress are able to differentially modulate the expression 
      of BDNF transcripts by possible different epigenetic mechanisms.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Ieraci, Alessandro
AU  - Ieraci A
AD  - Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento 
      di Scienze Farmacologiche e Biomolecolari and CEND, Universita degli Studi di 
      Milano, Milano, Italy.
FAU - Mallei, Alessandra
AU  - Mallei A
AD  - Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento 
      di Scienze Farmacologiche e Biomolecolari and CEND, Universita degli Studi di 
      Milano, Milano, Italy.
FAU - Musazzi, Laura
AU  - Musazzi L
AD  - Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento 
      di Scienze Farmacologiche e Biomolecolari and CEND, Universita degli Studi di 
      Milano, Milano, Italy.
FAU - Popoli, Maurizio
AU  - Popoli M
AD  - Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento 
      di Scienze Farmacologiche e Biomolecolari and CEND, Universita degli Studi di 
      Milano, Milano, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150415
PL  - United States
TA  - Hippocampus
JT  - Hippocampus
JID - 9108167
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/drug effects/*metabolism
MH  - Epigenesis, Genetic/drug effects/*physiology
MH  - Hippocampus/drug effects/*metabolism
MH  - Histone Deacetylase Inhibitors/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Motor Activity/drug effects/*physiology
MH  - RNA, Messenger/drug effects/metabolism
MH  - Restraint, Physical/*physiology
MH  - Stress, Psychological/*metabolism/prevention & control
OTO - NOTNLM
OT  - BDNF exons
OT  - HDAC
OT  - hippocampus
OT  - restraint stress
OT  - running
EDAT- 2015/03/31 06:00
MHDA- 2016/08/30 06:00
CRDT- 2015/03/31 06:00
PHST- 2015/03/17 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/08/30 06:00 [medline]
AID - 10.1002/hipo.22458 [doi]
PST - ppublish
SO  - Hippocampus. 2015 Nov;25(11):1380-92. doi: 10.1002/hipo.22458. Epub 2015 Apr 15.