PMID- 25821472
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150330
LR  - 20200930
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2015
DP  - 2015
TI  - Comparisons of mouse mesenchymal stem cells in primary adherent culture of 
      compact bone fragments and whole bone marrow.
PG  - 708906
LID - 10.1155/2015/708906 [doi]
LID - 708906
AB  - The purification of mouse bone marrow mesenchymal stem cells (BMSCs) by using the 
      standard method of whole bone marrow adherence to plastic still remains 
      ineffective. An increasing number of studies have indicated compact bone as an 
      alternative source of BMSCs. We isolated BMSCs from cultured compact bone 
      fragments and investigated the proliferative capacity, surface immunophenotypes, 
      and osteogenic and adipogenic differentiations of the cells after the first 
      trypsinization. The fragment culture was based on the fact that BMSCs were 
      assembled in compact bones. Thus, the procedure included flushing bone marrow out 
      of bone cavity and culturing the fragments without any collagenase digestion. The 
      cell yield from cultured fragments was slightly less than that from cultured bone 
      marrow using the same bone quantity. However, the trypsinized cells from cultured 
      fragments exhibited significantly higher proliferation and were accompanied with 
      more CD90 and CD44 expressions and less CD45 expression. The osteogenic and 
      adipogenic differentiation capacity of cells from cultured fragments were better 
      than those of cells from bone marrow. The directly adherent culture of compact 
      bone is suitable for mouse BMSC isolation, and more BMSCs with potentially 
      improved proliferation capacity can be obtained in the primary culture.
FAU - Cai, Yiting
AU  - Cai Y
AD  - Family Planning Research Institute, Tongji Medical College, Huazhong University 
      of Science and Technology, Wuhan 430030, China.
FAU - Liu, Tianshu
AU  - Liu T
AD  - Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan 
      430060, China.
FAU - Fang, Fang
AU  - Fang F
AD  - Family Planning Research Institute, Tongji Medical College, Huazhong University 
      of Science and Technology, Wuhan 430030, China.
FAU - Xiong, Chengliang
AU  - Xiong C
AD  - Family Planning Research Institute, Tongji Medical College, Huazhong University 
      of Science and Technology, Wuhan 430030, China ; Center for Reproductive 
      Medicine, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan 430010, China.
FAU - Shen, Shiliang
AU  - Shen S
AD  - Zhong Shen Bioscience Inc., Biolake B3-1, 666 Gaoxin Road, East Lake Hi-Tech 
      Zone, Wuhan 430000, China.
LA  - eng
PT  - Journal Article
DEP - 20150303
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC4363588
EDAT- 2015/03/31 06:00
MHDA- 2015/03/31 06:01
PMCR- 2015/03/03
CRDT- 2015/03/31 06:00
PHST- 2014/09/29 00:00 [received]
PHST- 2015/01/22 00:00 [revised]
PHST- 2015/01/29 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/03/31 06:01 [medline]
PHST- 2015/03/03 00:00 [pmc-release]
AID - 10.1155/2015/708906 [doi]
PST - ppublish
SO  - Stem Cells Int. 2015;2015:708906. doi: 10.1155/2015/708906. Epub 2015 Mar 3.