PMID- 25821591
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150330
LR  - 20200930
IS  - 2090-004X (Print)
IS  - 2090-0058 (Electronic)
IS  - 2090-004X (Linking)
VI  - 2015
DP  - 2015
TI  - Retinal Neurodegeneration in db/db Mice at the Early Period of Diabetes.
PG  - 757412
LID - 10.1155/2015/757412 [doi]
LID - 757412
AB  - Purpose. To describe both the functional and pathological alternations in 
      neurosensory retina in a murine model of spontaneous type 2 diabetes (db/db 
      mouse). Methods. db/db (BKS/DB-/-) mice and heterozygous littermates (as control 
      group) at various ages (12, 16, 20, 24, and 28 weeks) were inspected with pattern 
      electroretinogram (PERG), fundus fluorescein angiography (FFA), and optical 
      coherence tomography (OCT). Histological markers of neuroinflammation (IBA-1 and 
      F4/80) were evaluated by immunohistochemistry. In addition, levels of retinal 
      ganglion cell death were measured by terminal dUTP nick-end labeling (TUNEL). 
      Results. Significant alternations of PERG responses and increased retinal 
      ganglion cells (RGCs) apoptosis were observed in diabetic db/db mice for 20-week 
      period when compared with control group. IBA-1 and F4/80 expression in 
      microglia/macrophages became evidently for 24-week period, thus supporting the 
      PERG findings. Furthermore, obvious thinning of nasal and dorsal retina in 
      28-week-old db/db mice was also revealed by OCT. No visible retinal microvascular 
      changes were detected by FFA throughout the experiments on db/db mice. 
      Conclusions. Diabetic retina underwent neurodegenerative changes in db/db mice, 
      which happened at retinal ganglion cell layer and inner nuclear layer. But there 
      was no obvious abnormality in retinal vasculature on db/db mice.
FAU - Yang, Qin
AU  - Yang Q
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Xu, Yidan
AU  - Xu Y
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Xie, Ping
AU  - Xie P
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Cheng, Haixia
AU  - Cheng H
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Song, Qinglu
AU  - Song Q
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Su, Tu
AU  - Su T
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Yuan, Songtao
AU  - Yuan S
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
FAU - Liu, Qinghuai
AU  - Liu Q
AD  - Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu 210029, China.
LA  - eng
PT  - Journal Article
DEP - 20150302
PL  - United States
TA  - J Ophthalmol
JT  - Journal of ophthalmology
JID - 101524199
PMC - PMC4363796
EDAT- 2015/03/31 06:00
MHDA- 2015/03/31 06:01
PMCR- 2015/03/02
CRDT- 2015/03/31 06:00
PHST- 2014/10/30 00:00 [received]
PHST- 2015/01/20 00:00 [revised]
PHST- 2015/02/02 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2015/03/31 06:01 [medline]
PHST- 2015/03/02 00:00 [pmc-release]
AID - 10.1155/2015/757412 [doi]
PST - ppublish
SO  - J Ophthalmol. 2015;2015:757412. doi: 10.1155/2015/757412. Epub 2015 Mar 2.