PMID- 25822694
OWN - NLM
STAT- MEDLINE
DCOM- 20160411
LR  - 20181113
IS  - 1439-6327 (Electronic)
IS  - 1439-6319 (Linking)
VI  - 115
IP  - 8
DP  - 2015 Aug
TI  - The impact of intensified training with a high or moderate carbohydrate feeding 
      strategy on resting and exercise-induced oxidative stress.
PG  - 1757-67
LID - 10.1007/s00421-015-3162-4 [doi]
AB  - PURPOSE: This study investigated the impact of intensified training (IT) and 
      carbohydrate (CHO) supplementation on resting and exercise-induced oxidative 
      stress. METHODS: Male cyclists (n = 13, mean +/- SD: age 25 +/- 6 years; [Formula: 
      see text] 72 +/- 5 ml/kg/min) undertook two 9 day periods of endurance-based IT. In 
      a counter-balanced, crossover and double-blinded study design, participants 
      completed IT whilst ingesting high (H-CHO) or moderate (M-CHO) CHO beverages 
      before (H-CHO: 24 g vs. M-CHO: 2 g), during (H-CHO: 60 g/h vs. M-CHO: 20 g/h) and 
      after training sessions (H-CHO: 44 g vs. M-CHO: 10 g). Participants completed 
      fasted performance trials without CHO on days 2, 6 and 10. Blood samples were 
      taken before and immediately after exercise to assess plasma oxidative stress. 
      RESULTS: Resting thiol (-SH) and catalase (CAT) activities decreased following 6 
      days of IT, independent of CHO condition [-SH (muM oxidised NADPH): H-CHO-14.0 +/- 
      18.8, M-CHO-20.4 +/- 20.3 and CAT (nmol/min/ml): H-CHO 12.5 +/- 12.5, M-CHO 6.0 +/- 
      4.5; all p < 0.05]. Resting total antioxidant capacity (TAC) was reduced after IT 
      in M-CHO. All exercise bouts elicited significant increases in CAT, TAC, protein 
      carbonylation (PC) and lipid hydroperoxides (LOOH), independent of CHO condition 
      (p < 0.05). The magnitude of increase in PC and LOOH was greater on days 6 and 10 
      compared to day 2 in both conditions. CONCLUSIONS: Short-term IT caused 
      reductions in resting antioxidant capacity in trained cyclists. Exercise-induced 
      increases in PC and LOOH were exaggerated as a result of IT; however, these 
      responses were independent of carbohydrate intake before, during and after the 
      preceding IT sessions.
FAU - Wadley, Alex J
AU  - Wadley AJ
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Leicestershire, LE11 3TU, England, UK, a.wadley@worc.ac.uk.
FAU - Killer, Sophie C
AU  - Killer SC
FAU - Svendsen, Ida S
AU  - Svendsen IS
FAU - Gleeson, Michael
AU  - Gleeson M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20150331
PL  - Germany
TA  - Eur J Appl Physiol
JT  - European journal of applied physiology
JID - 100954790
RN  - 0 (Antioxidants)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Sulfhydryl Compounds)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Adult
MH  - Affect/drug effects
MH  - Anaerobic Threshold
MH  - Antioxidants/metabolism
MH  - Bicycling/*physiology
MH  - Catalase/blood
MH  - Cross-Over Studies
MH  - Dietary Carbohydrates/*pharmacology
MH  - Double-Blind Method
MH  - Humans
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - Oxidative Stress/*drug effects
MH  - Physical Conditioning, Human/*methods
MH  - Protein Carbonylation
MH  - Rest/*physiology
MH  - Sulfhydryl Compounds/blood
MH  - Young Adult
EDAT- 2015/03/31 06:00
MHDA- 2016/04/12 06:00
CRDT- 2015/03/31 06:00
PHST- 2014/12/10 00:00 [received]
PHST- 2015/03/24 00:00 [accepted]
PHST- 2015/03/31 06:00 [entrez]
PHST- 2015/03/31 06:00 [pubmed]
PHST- 2016/04/12 06:00 [medline]
AID - 10.1007/s00421-015-3162-4 [doi]
PST - ppublish
SO  - Eur J Appl Physiol. 2015 Aug;115(8):1757-67. doi: 10.1007/s00421-015-3162-4. Epub 
      2015 Mar 31.