PMID- 25823643
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20191210
IS  - 1873-5835 (Electronic)
IS  - 0145-2126 (Linking)
VI  - 39
IP  - 5
DP  - 2015 May
TI  - Standardized fluorescence in situ hybridization testing based on an appropriate 
      panel of probes more effectively identifies common cytogenetic abnormalities in 
      myelodysplastic syndromes than conventional cytogenetic analysis: a multicenter 
      prospective study of 2302 patients in China.
PG  - 530-5
LID - S0145-2126(15)00038-7 [pii]
LID - 10.1016/j.leukres.2015.02.005 [doi]
AB  - In an attempt to establish the advantages of fluorescence in situ hybridization 
      (FISH) studies over conventional cytogenetic (CC) analysis, a total of 2302 de 
      novo MDS patients from 31 Chinese institutions were prospectively selected in the 
      present study for both CC and standardized FISH analysis for +8, -7/7q-, -5/5q-, 
      20q- and-Y chromosomal abnormalities. CC analysis was successful in 94.0% of the 
      patients; of these patients, 35.9% of the cases were abnormal. FISH analysis was 
      successful in all 2302 patients and detected at least one type of common 
      cytogenetic abnormality in 42.7% of the cases. The incidences of +8, -7/7q-, 
      -5/5q-, 20q- and-Y chromosomal abnormalities by FISH were 4.1% to 8.7% higher 
      than those by CC. FISH identified abnormalities in 23.6% of the patients 
      exhibiting normal CC results and revealed that 20.7% of the patients with 
      adequate normal metaphases (>/=20) had abnormal clones. FISH identified cytogenetic 
      abnormalities in 50.4% of the patients with failed CC analysis. In summary, our 
      multicenter studies emphasised and confirmed the importance of applying 
      standardized FISH testing based on an appropriate panel of probes to detect 
      common cytogenetic abnormalities in Chinese de novo MDS patients, particularly 
      those with normal or failed CC results.
CI  - Copyright (c) 2015. Published by Elsevier Ltd.
FAU - Lai, Yue-Yun
AU  - Lai YY
AD  - Peking University People's Hospital, Peking University Institute of Hematology, 
      No. 11 Xizhimen South Street, Beijing, China.
FAU - Huang, Xiao-Jun
AU  - Huang XJ
AD  - Peking University People's Hospital, Peking University Institute of Hematology, 
      No. 11 Xizhimen South Street, Beijing, China. Electronic address: 
      xjhrm@medmail.com.cn.
FAU - Li, Juan
AU  - Li J
AD  - Department of Hematology, First Affiliated Hospital of Zhongshan University, 
      Guangzhou, China.
FAU - Zou, Ping
AU  - Zou P
AD  - Department of Hematology, Wuhan Union Hospital, Wuhan, China.
FAU - Xu, Ze-Feng
AU  - Xu ZF
AD  - Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of 
      Medical Sciences and Peking Union Medical College, Tianjin, China.
FAU - Sun, Hui
AU  - Sun H
AD  - Department of Hematology, First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Shao, Zong-Hong
AU  - Shao ZH
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Zhou, Dao-Bin
AU  - Zhou DB
AD  - Department of Hematology, Peking Union Medical College Hospital, Beijing, China.
FAU - Chen, Fang-Ping
AU  - Chen FP
AD  - Department of Hematology, Xiangya Hospital of Central-South University, Changsha, 
      China.
FAU - Liu, Zhuo-Gang
AU  - Liu ZG
AD  - Department of Hematology, Shengjing Hospital, China Medical University, Shenyang, 
      China.
FAU - Zhu, Huan-Ling
AU  - Zhu HL
AD  - Department of Hematology, West China Hospital of Sichuan University, Chengdu, 
      China.
FAU - Wu, De-Pei
AU  - Wu DP
AD  - Department of Hematology, First Affiliated Hospital of Soochow University, 
      Suzhou, China.
FAU - Wang, Chun
AU  - Wang C
AD  - Department of Hematology, Shanghai First People's Hospital, Shanghai, China.
FAU - Zhang, Yin
AU  - Zhang Y
AD  - Department of Hematology, Henan Province People's Hospital, Zhengzhou, China.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Hematology, First Affiliated Hospital, China Medical University, 
      Shenyang, China.
FAU - Hou, Ming
AU  - Hou M
AD  - Department of Hematology, Qilu Hospital of Shandong University, Jinan, China.
FAU - Du, Xin
AU  - Du X
AD  - Department of Hematology, Guangdong Provincial People's Hospital, Guangzhou, 
      China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Hematology, Shandong Provincial Hospital of Shandong University, 
      Jinan, China.
FAU - Li, Wei
AU  - Li W
AD  - Department of Hematology, First Affiliated Hospital of Jilin University, 
      Changchun, China.
FAU - Lai, Yong-Rong
AU  - Lai YR
AD  - Department of Hematology, First Affiliated Hospital, Guangxi Medical University, 
      Nanning, China.
FAU - Zhou, Jin
AU  - Zhou J
AD  - Department of Hematology, First Clinical College of Harbin Medical University, 
      Harbin, China.
FAU - Zhou, Yu-Hong
AU  - Zhou YH
AD  - Department of Hematology, Zhejiang Provincial Hospital of Traditional Chinese 
      Medicine, Hangzhou, China.
FAU - Fang, Mei-Yun
AU  - Fang MY
AD  - Department of Hematology, First Affiliated Hospital of Dalian Medical University, 
      Dalian, China.
FAU - Qiu, Lin
AU  - Qiu L
AD  - Harbin Institute of Hematology and Oncology, Harbin, China.
FAU - Wang, Xiao-Min
AU  - Wang XM
AD  - Department of Hematology, People's Hospital of Xinjiang Uygur Autonomous Region, 
      Urumqi, China.
FAU - Zhang, Guang-Sen
AU  - Zhang GS
AD  - Department of Hematology, Second Xiangya Hospital of Central-South University, 
      Changsha, China.
FAU - Jiang, Ming
AU  - Jiang M
AD  - Department of Hematology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, China.
FAU - Liang, Ying-Min
AU  - Liang YM
AD  - Department of Hematology, Tangdu Hospital, Fourth Military Medical University, 
      Xian, China.
FAU - Zhang, Lian-Sheng
AU  - Zhang LS
AD  - Department of Hematology, Second Hospital of Lanzhou University, Lanzhou, China.
FAU - Chen, Xie-Qun
AU  - Chen XQ
AD  - Department of Hematology, Xijing Hospital Affiliated to the Fourth Military 
      Medical University, Xian, China.
FAU - Bai, Hai
AU  - Bai H
AD  - Department of Hematology, Lanzhou General Hospital of Lanzhou Command, Lanzhou, 
      China.
FAU - Lin, Jin-Ying
AU  - Lin JY
AD  - Department of Hematology, People's Hospital of Guangxi Zhuang Autonomous Region, 
      Nanning, China.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20150218
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - China
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis/*methods/standards
MH  - Female
MH  - *Fluorescent Dyes
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods/standards
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*diagnosis/genetics
MH  - Predictive Value of Tests
MH  - Prospective Studies
MH  - Reference Standards
MH  - Young Adult
OTO - NOTNLM
OT  - ChiCTR-ONRC-11001709
OT  - Cytogenetics
OT  - Fluorescence in situ hybridization
OT  - Myelodysplastic syndrome
EDAT- 2015/04/01 06:00
MHDA- 2015/08/04 06:00
CRDT- 2015/04/01 06:00
PHST- 2014/09/26 00:00 [received]
PHST- 2015/01/22 00:00 [revised]
PHST- 2015/02/10 00:00 [accepted]
PHST- 2015/04/01 06:00 [entrez]
PHST- 2015/04/01 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
AID - S0145-2126(15)00038-7 [pii]
AID - 10.1016/j.leukres.2015.02.005 [doi]
PST - ppublish
SO  - Leuk Res. 2015 May;39(5):530-5. doi: 10.1016/j.leukres.2015.02.005. Epub 2015 Feb 
      18.