PMID- 2582404
OWN - NLM
STAT- MEDLINE
DCOM- 19850717
LR  - 20131121
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 179
IP  - 4-5
DP  - 1985 Mar
TI  - Effects of aphidicolin and alpha-amanitin on visualization of acridine orange 
      binding to DNA in rat astrocytoma C6 cells.
PG  - 499-503
AB  - The purpose of this study was to examine effects of aphidicolin and 
      alpha-amanitin on visualization of acridine orange (AO) binding to DNA in rat 
      astrocytoma C6 cells and to discuss briefly the significance of AO chromatin 
      interaction products. Aphidicolin inhibited DNA synthesis but percentage of AO 
      positive cells was approximately 60% of that of the untreated control cells. 
      alpha-Amanitin caused a slight inhibition of RNA synthesis and 3H-uridine 
      incorporation in the treated cells was about 64% of that of the untreated cells, 
      whereas a distinct decrease of the number of AO positive cell nuclei was 
      observed. The results suggest that activity of RNA polymerase II and mRNA 
      synthesis is mainly concerned in visualization of AO chromatin interaction 
      products.
FAU - Hara, H
AU  - Hara H
FAU - Moriki, T
AU  - Moriki T
FAU - Hiroi, M
AU  - Hiroi M
FAU - Yamane, T
AU  - Yamane T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Amanitins)
RN  - 0 (Diterpenes)
RN  - 38966-21-1 (Aphidicolin)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Amanitins/*pharmacology
MH  - Animals
MH  - Aphidicolin
MH  - Astrocytoma
MH  - Cells, Cultured
MH  - DNA/biosynthesis/*metabolism
MH  - Diterpenes/*pharmacology
MH  - RNA/biosynthesis
MH  - Rats
MH  - Staining and Labeling
EDAT- 1985/03/01 00:00
MHDA- 1985/03/01 00:01
CRDT- 1985/03/01 00:00
PHST- 1985/03/01 00:00 [pubmed]
PHST- 1985/03/01 00:01 [medline]
PHST- 1985/03/01 00:00 [entrez]
PST - ppublish
SO  - Pathol Res Pract. 1985 Mar;179(4-5):499-503.