PMID- 25826065
OWN - NLM
STAT- MEDLINE
DCOM- 20160609
LR  - 20150401
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 32
IP  - 2
DP  - 2015 Apr
TI  - Histological and Histochemical Study of the Hepatopancreas of Two Estuarine Crab 
      Species, Cyrtograpsus angulatus and Neohelice granulata (Grapsoidea, Varunidae): 
      Influence of Environmental Salinity.
PG  - 163-70
LID - 10.2108/zs140133 [doi]
AB  - The aim of this study was to analyze the histology and the histochemical 
      distribution of glycoproteins (GPs) and lipids of the hepatopancreas of 
      Cyrtograpsus angulatus and Neohelice granulata acclimated to salinities of 10 psu 
      (hyperregulation) and 35 psu (osmoconformation). Sections of the hepatopancreas 
      of adult male crabs were treated with hematoxylin-eosin; Masson trichrome; Alcian 
      Blue (pHs 2.8, 1.0, 0.5); Toluidine Blue (pHs 5.6, 4.2); periodic acid Schiff; 
      Sudan Black and Red. At salinity 35 psu, the hepatopancreas of both species 
      exhibited typical histological features, whereas at salinity 10 psu, detachment 
      of the basal lamina, desquamated epithelium, disrupted brush border, loss of 
      intercellular cohesion, hypertrophied tubular lumen, and hemolymph infiltration 
      between cells were observed in some zones. Resorptive cells (R-cells) and 
      vacuoles of blister-like cells (B-cells) of both species show a higher glycogen 
      content at 35 psu than at 10 psu. At lower salinities, the cytoplasm of the 
      different cell types evidence higher contents of carboxylated GPs in N. granulata 
      and of su If at ed GPs in C. angulatus. At both salinities, and at the two pHs in 
      N. granulata and at pH 5.6 in C. angulatus, the brush border, the vacuoles of 
      B-cells and the peritrophic membrane show metachromasia. R-cell vacuoles and the 
      cytoplasm of all cell types--except for the E-cells--at all salinities in both 
      species show abundant lipid droplets. The results of the present study contribute 
      significant data to the histophysiology of crustacean decapods, favoring the 
      comprehension of the complex adjustment mechanisms facing saline stress in 
      euryhaline crabs.
FAU - Longo, Maria Victoria
AU  - Longo MV
AD  - Institute of Marine and Coastal Research, National Council of Scientific and 
      Technical Research - National University of Mar del Plata. Funes 3250, Mar del 
      Plata (7600), Buenos Aires, Argentina.
FAU - Diaz, Alcira Ofelia
AU  - Diaz AO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 0 (Glycoproteins)
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Brachyura/*physiology
MH  - Ecosystem
MH  - Glycoproteins/chemistry/metabolism
MH  - Hepatopancreas/cytology/*physiology
MH  - Lipids/chemistry
MH  - Male
MH  - *Salinity
MH  - Seawater/*chemistry
OTO - NOTNLM
OT  - euryhaline crabs
OT  - glycoproteins
OT  - hepatopancreas
OT  - histology
OT  - lipids
EDAT- 2015/04/01 06:00
MHDA- 2016/06/10 06:00
CRDT- 2015/04/01 06:00
PHST- 2015/04/01 06:00 [entrez]
PHST- 2015/04/01 06:00 [pubmed]
PHST- 2016/06/10 06:00 [medline]
AID - 10.2108/zs140133 [doi]
PST - ppublish
SO  - Zoolog Sci. 2015 Apr;32(2):163-70. doi: 10.2108/zs140133.