PMID- 25827488
OWN - NLM
STAT- MEDLINE
DCOM- 20150914
LR  - 20200325
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 594
DP  - 2015 May 6
TI  - Monocarboxylate transporter-dependent mechanism confers resistance to oxygen- and 
      glucose-deprivation injury in astrocyte-neuron co-cultures.
PG  - 99-104
LID - S0304-3940(15)00255-4 [pii]
LID - 10.1016/j.neulet.2015.03.062 [doi]
AB  - Hypoxic and low-glucose stressors contribute to neuronal death in many brain 
      diseases. Astrocytes are anatomically well-positioned to shield neurons from 
      hypoxic injury. During hypoxia/ischemia, lactate released from astrocytes is 
      taken up by neurons and stored for energy. This process is mediated by 
      monocarboxylate transporters (MCTs) in the central nervous system. In the present 
      study, we investigated the ability of astrocytes to protect neurons from oxygen- 
      and glucose-deprivation (OGD) injury via an MCT-dependent mechanism in vitro. 
      Primary cultures of neurons, astrocytes, and astrocytes-neurons derived from rat 
      hippocampus were subjected to OGD, MCT inhibition with small interfering (si)RNA. 
      Cell survival and expression of MCT4, MCT2, glial fibrillary acidic protein, and 
      neuronal nuclear antigen were evaluated. OGD significantly increased cell death 
      in neuronal cultures and up-regulated MCT4 expression in astrocyte cultures, but 
      no increased cell death was observed in neuron-astrocyte co-cultures or astrocyte 
      cultures. However, neuronal cell death in co-cultures was increased by exposure 
      to MCT4- or MCT2-specific siRNA, and this effect was attenuated by the addition 
      of lactate into the extracellular medium of neuronal cultures prior to OGD. These 
      findings demonstrate that resistance to OGD injury in astrocyte-neuron 
      co-cultures occurs via an MCT-dependent mechanism.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Gao, Chen
AU  - Gao C
AD  - Department of Neurosurgery, AnNing Branch Hospital, Lanzhou General Hospital, 
      Lanzhou Command, PLA, Lanzhou, Gansu Province 730070, China.
FAU - Zhou, Liya
AU  - Zhou L
AD  - Electrophysiology Examining Room, Department of Neurology, People's Hospital of 
      Gansu Province, Lanzhou, Gansu Province 730000, China.
FAU - Zhu, Wenxia
AU  - Zhu W
AD  - Department of Traumatology, AnNing Branch Hospital, Lanzhou General Hospital, 
      Lanzhou Command, PLA, Lanzhou, Gansu Province 730070, China.
FAU - Wang, Hongyun
AU  - Wang H
AD  - Department of Neurosurgery, AnNing Branch Hospital, Lanzhou General Hospital, 
      Lanzhou Command, PLA, Lanzhou, Gansu Province 730070, China.
FAU - Wang, Ruijuan
AU  - Wang R
AD  - Department of Neurosurgery, AnNing Branch Hospital, Lanzhou General Hospital, 
      Lanzhou Command, PLA, Lanzhou, Gansu Province 730070, China.
FAU - He, Yunfei
AU  - He Y
AD  - Department of Traumatology, AnNing Branch Hospital, Lanzhou General Hospital, 
      Lanzhou Command, PLA, Lanzhou, Gansu Province 730070, China.
FAU - Li, Zhiyun
AU  - Li Z
AD  - Department of Neurosurgery, Lanzhou General Hospital, Lanzhou Command, PLA, South 
      of Binhe Road, No. 333, Lanzhou, Gansu Province 730050, China. Electronic 
      address: Lizhiyun890@163.com.
LA  - eng
PT  - Journal Article
DEP - 20150328
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Monocarboxylic Acid Transporters)
RN  - 0 (Muscle Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Rbfox3 protein, rat)
RN  - 0 (Slc16a3 protein, rat)
RN  - 0 (Slc16a7 protein, rat)
RN  - 33X04XA5AT (Lactic Acid)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Antigens, Nuclear/metabolism
MH  - Astrocytes/cytology/drug effects/*metabolism
MH  - Cell Hypoxia
MH  - Cell Survival
MH  - Coculture Techniques
MH  - Embryo, Mammalian/cytology
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glucose/*deficiency
MH  - Hippocampus/cytology
MH  - Lactic Acid/pharmacology
MH  - Monocarboxylic Acid Transporters/genetics/*metabolism
MH  - Muscle Proteins/genetics/*metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Oxygen/*metabolism
MH  - RNA Interference
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Astrocyte
OT  - Co-culture
OT  - Glucose deprivation
OT  - Monocarboxylate transporters
OT  - Oxygen deprivation
EDAT- 2015/04/02 06:00
MHDA- 2015/09/15 06:00
CRDT- 2015/04/02 06:00
PHST- 2015/02/08 00:00 [received]
PHST- 2015/03/26 00:00 [revised]
PHST- 2015/03/27 00:00 [accepted]
PHST- 2015/04/02 06:00 [entrez]
PHST- 2015/04/02 06:00 [pubmed]
PHST- 2015/09/15 06:00 [medline]
AID - S0304-3940(15)00255-4 [pii]
AID - 10.1016/j.neulet.2015.03.062 [doi]
PST - ppublish
SO  - Neurosci Lett. 2015 May 6;594:99-104. doi: 10.1016/j.neulet.2015.03.062. Epub 
      2015 Mar 28.