PMID- 25828589
OWN - NLM
STAT- MEDLINE
DCOM- 20160503
LR  - 20220317
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 24
IP  - 6
DP  - 2015 Jun
TI  - In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator 
      of oestrogen-stimulated keratinocyte migration.
PG  - 478-80
LID - 10.1111/exd.12713 [doi]
AB  - It is known that oestrogen influences skin wound healing by modulating the 
      inflammatory response, cytokine expression and extracellular matrix deposition; 
      accelerating re-epithelialization; and stimulating angiogenesis. To identify 
      novel proteins associated with effects of oestrogen on keratinocyte, stable 
      isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry 
      was performed. Using SILAC, quantification of 1085 proteins was achieved. Among 
      these proteins, 60 proteins were upregulated and 32 proteins were downregulated. 
      Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) 
      has been further evaluated for its role in the effect of oestrogen on 
      keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated 
      keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to 
      significantly accelerate the rate of HaCaT cell migration. To determine whether 
      HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with 
      HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. 
      Collectively, these experiments demonstrate that HMGB1 is a novel downstream 
      mediator of oestrogen-stimulated keratinocyte migration.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Shin, Jung U
AU  - Shin JU
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, Korea.
FAU - Noh, Ji Yeon
AU  - Noh JY
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, Korea.
FAU - Lee, Ju Hee
AU  - Lee JH
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, Korea.
FAU - Lee, Won Jai
AU  - Lee WJ
AD  - Department of Plastic and Reconstructive Surgery, Severance Hospital, Institute 
      for Human Tissue Restoration, Yonsei University College of Medicine, Seoul, 
      Korea.
FAU - Yoo, Jong Shin
AU  - Yoo JS
AD  - Division of Mass Spectrometry, Korea Basic Science Institute, Ochang-Myun, 
      Cheongwon-Gun, Korea.
AD  - GRAST, Chungnam National University, Daejeon, Korea.
FAU - Kim, Jin Young
AU  - Kim JY
AD  - Division of Mass Spectrometry, Korea Basic Science Institute, Ochang-Myun, 
      Cheongwon-Gun, Korea.
AD  - GRAST, Chungnam National University, Daejeon, Korea.
FAU - Kim, Hyeran
AU  - Kim H
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, Korea.
FAU - Jung, Inhee
AU  - Jung I
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, Korea.
FAU - Jin, Shan
AU  - Jin S
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, Korea.
AD  - Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of 
      Medicine, Seoul Korea.
FAU - Lee, Kwang Hoon
AU  - Lee KH
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, Korea.
AD  - Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of 
      Medicine, Seoul Korea.
LA  - eng
PT  - Letter
DEP - 20150427
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Estrogens)
RN  - 0 (HMGB1 Protein)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Cell Line
MH  - Cell Movement/*drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Estrogens/*pharmacology
MH  - Gene Expression Regulation/drug effects/genetics
MH  - Gene Knockdown Techniques
MH  - HMGB1 Protein/drug effects/genetics/*physiology
MH  - Humans
MH  - Keratinocytes/cytology/*drug effects/physiology
MH  - Proteomics/*methods
MH  - RNA, Small Interfering/genetics/pharmacology
MH  - Signal Transduction/drug effects/physiology
MH  - Time Factors
OTO - NOTNLM
OT  - HMGB1
OT  - estrogen
OT  - keratinocyte
EDAT- 2015/04/02 06:00
MHDA- 2016/05/04 06:00
CRDT- 2015/04/02 06:00
PHST- 2015/03/26 00:00 [accepted]
PHST- 2015/04/02 06:00 [entrez]
PHST- 2015/04/02 06:00 [pubmed]
PHST- 2016/05/04 06:00 [medline]
AID - 10.1111/exd.12713 [doi]
PST - ppublish
SO  - Exp Dermatol. 2015 Jun;24(6):478-80. doi: 10.1111/exd.12713. Epub 2015 Apr 27.