PMID- 2582968
OWN - NLM
STAT- MEDLINE
DCOM- 19891229
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 10
IP  - 6
DP  - 1989 Nov
TI  - Separation of hepatocytes of different acinar zones by flow cytometry.
PG  - 772-8
AB  - Hepatocytes in the proximal (zone 1) and distal (zone 3) regions of the liver 
      acinus are selectively stained by perfusion of the isolated rat liver with 0.2-20 
      microM acridine orange (AO). After 10-60 min of anterograde perfusion, AO 
      fluorescence is visible in zone 1 cells, whereas retrograde perfusion stains 
      cells of zone 3. In this paper, we describe a technique to isolate a mixed 
      population of fluorescent and nonfluorescent hepatocytes (cells from all acinar 
      zones, which do not loose the zone specific AO labeling) and to separate these 
      cells according to their zonal origin by fluorescence activated cell sorting. The 
      zonal populations obtained were either fluorescent or nonfluorescent (purity 
      greater than 95%). Separated cell fractions differed in their enzyme content (5' 
      nucleotidase, succinate-dehydrogenase, beta-glucuronidase). An unidentified AO 
      metabolite, which is not found in bile after retrograde perfusion (not formed in 
      zone 3 cells), is also absent after retrograde perfusion in sorted fluorescent 
      cells (zone 3 cells), indicating zonal purity of sorted cells.
FAU - Thalhammer, T
AU  - Thalhammer T
AD  - Department of General and Experimental Pathology, University of Vienna, Austria.
FAU - Gessl, A
AU  - Gessl A
FAU - Braakman, I
AU  - Braakman I
FAU - Graf, J
AU  - Graf J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Biliary Tract/metabolism
MH  - Cell Separation
MH  - Flow Cytometry/methods
MH  - Liver/*cytology/metabolism
MH  - Male
MH  - Rats
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
AID - 10.1002/cyto.990100615 [doi]
PST - ppublish
SO  - Cytometry. 1989 Nov;10(6):772-8. doi: 10.1002/cyto.990100615.