PMID- 2583380
OWN - NLM
STAT- MEDLINE
DCOM- 19900111
LR  - 20190515
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 38
IP  - 12
DP  - 1989 Dec
TI  - Rapid detection of IDDM susceptibility with HLA-DQ beta-alleles as markers.
PG  - 1617-22
AB  - The allelic forms of the human leukocyte antigen (HLA)-DQ beta-chain (DQB1) have 
      been recognized as the best markers of insulin-dependent diabetes mellitus (IDDM) 
      susceptibility. We describe a method that allows the recognition of these DQB1 
      alleles without the use of either allele-specific oligonucleotide probes or 
      radioactive material. This method determines these alleles by electrophoretically 
      separating restriction enzyme-generated fragments from the polymerase 
      chain-reaction-amplified second exon of the HLA-DQB1 gene, which encodes the 
      first domain of the protein chain. This digestion method, which is simpler and 
      more rapid than the previously adopted hybridization method, is described in 
      detail to enable individuals at any clinical laboratory to quickly ascertain IDDM 
      susceptibility.
FAU - Trucco, G
AU  - Trucco G
AD  - Department of Pathology, University of Pittsburgh, School of Medicine, 
      Pennsylvania.
FAU - Fritsch, R
AU  - Fritsch R
FAU - Giorda, R
AU  - Giorda R
FAU - Trucco, M
AU  - Trucco M
LA  - eng
GR  - AI-23963/AI/NIAID NIH HHS/United States
GR  - CA-44977/CA/NCI NIH HHS/United States
GR  - DK-24021/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Biomarkers)
RN  - 0 (Codon)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - Biomarkers/*analysis
MH  - Codon/genetics
MH  - Diabetes Mellitus, Type 1/*genetics/immunology
MH  - Disease Susceptibility
MH  - HLA-DQ Antigens/analysis/*genetics
MH  - Humans
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction
EDAT- 1989/12/01 00:00
MHDA- 1989/12/01 00:01
CRDT- 1989/12/01 00:00
PHST- 1989/12/01 00:00 [pubmed]
PHST- 1989/12/01 00:01 [medline]
PHST- 1989/12/01 00:00 [entrez]
AID - 10.2337/diab.38.12.1617 [doi]
PST - ppublish
SO  - Diabetes. 1989 Dec;38(12):1617-22. doi: 10.2337/diab.38.12.1617.