PMID- 25838250
OWN - NLM
STAT- MEDLINE
DCOM- 20150720
LR  - 20171213
IS  - 2038-2529 (Electronic)
IS  - 0300-8916 (Linking)
VI  - 101
IP  - 2
DP  - 2015 Mar-Apr
TI  - Expression of CBY and methylation of CBY at promoter region in human laryngeal 
      squamous cell carcinoma.
PG  - 215-22
LID - 10.5301/tj.5000242 [doi]
AB  - AIMS AND BACKGROUND: Chibby (CBY), a beta-catenin binding partner, inhibits 
      Wnt/beta-catenin-mediated transcriptional activation by competing with Tcf/Lef 
      factors for beta-catenin binding and promoting the export of beta-catenin from nucleus 
      to cytoplasm. The regulatory effect of CBY in this signaling pathway suggests its 
      biological importance as a potential tumor suppressor gene. The purposes of this 
      study were to determine whether the expression of CBY was downregulated in human 
      laryngeal squamous cell carcinoma (LSCC) samples, the CpG sites of CBY at the 
      promoter region were methylated in these tumor samples, and reduced expression of 
      CBY was induced by methylation of CBY promoters. METHODS: CBY expression was 
      investigated by quantitative real-time PCR and immunohistochemistry in samples 
      from 36 LSCC patients. The methylation status of the CBY promoter was detected by 
      methylation-specific PCR. RESULTS: Compared with normal laryngeal mucosa, the 
      expression of CBY was downregulated in LSCC samples. The reduced CBY expression 
      rate was 58.33% (21/36) at the mRNA and 66.67% (24/36) at the protein level. The 
      promoters of CBY were methylated in 12/36 tumor samples, partially methylated in 
      5, and unmethylated in 19 samples. The methylation rate including incomplete 
      methylation was 47.22% (17/36) in tumor samples, while no methylation was 
      detected in normal laryngeal squamous epithelium. Compared with the unmethylated 
      group, the expression of CBY was significantly different in the methylated group 
      (p<0.05) but similar in the partially methylated group (p>0.05). 
      CONCLUSIONS: Our data indicate that CBY expression was downregulated in LSCC, 
      which may be partially caused by methylation of CBY promoters.
FAU - Ren, Gang
AU  - Ren G
AD  - Department of Otolaryngology, the First People's Hospital of Huzhou, Huzhou, 
      Zhejiang - China.
FAU - Zhao, De-An
AU  - Zhao DA
FAU - Xu, Jue
AU  - Xu J
FAU - Li, Bo-An
AU  - Li BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150320
PL  - United States
TA  - Tumori
JT  - Tumori
JID - 0111356
RN  - 0 (CBY1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/pathology
MH  - Carrier Proteins/genetics/*metabolism
MH  - CpG Islands/genetics
MH  - *DNA Methylation
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Nuclear Proteins/genetics/*metabolism
MH  - *Promoter Regions, Genetic/genetics
MH  - Real-Time Polymerase Chain Reaction
EDAT- 2015/04/04 06:00
MHDA- 2015/07/21 06:00
CRDT- 2015/04/04 06:00
PHST- 2014/09/16 00:00 [accepted]
PHST- 2015/04/04 06:00 [entrez]
PHST- 2015/04/04 06:00 [pubmed]
PHST- 2015/07/21 06:00 [medline]
AID - 2FC3AC2A-7D18-4F41-9561-340C4342E95C [pii]
AID - 10.5301/tj.5000242 [doi]
PST - ppublish
SO  - Tumori. 2015 Mar-Apr;101(2):215-22. doi: 10.5301/tj.5000242. Epub 2015 Mar 20.