PMID- 25838314
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20200220
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 94
IP  - 6
DP  - 2015 Jun
TI  - Induction of tissue- and stressor-specific kinomic responses in chickens exposed 
      to hot and cold stresses.
PG  - 1333-45
LID - 10.3382/ps/pev046 [doi]
AB  - Defining cellular responses at the level of global cellular kinase (kinome) 
      activity is a powerful approach to deciphering complex biology and identifying 
      biomarkers. Here we report on the development of a chicken-specific peptide array 
      and its application to characterizing kinome responses within the breast 
      (pectoralis major) and thigh (iliotibialis) muscles of poultry subject to 
      temperature stress to mimic conditions experienced by birds during commercial 
      transport. Breast and thigh muscles exhibited unique kinome profiles, 
      highlighting the distinct nature of these tissues. Against these distinct 
      backgrounds, tissue- and temperature-specific kinome responses were observed. In 
      breast, both cold and hot stresses activated calcium-dependent metabolic 
      adaptations. Also within breast, but specific to cold stress, was the activation 
      of ErbB signaling as well as dynamic patterns of phosphorylation of AMPK, a key 
      regulatory enzyme of metabolism. In thigh, cold stress induced responses 
      suggestive of the occurrence of tissue damage, including activation of innate 
      immune signaling pathways and tissue repair pathways (TGF-beta). In contrast, heat 
      stress in thigh activated pathways associated with protein and fat metabolism 
      through adipocytokine and mammalian target of rapamycin (mTOR) signaling. 
      Defining the responses of these tissues to these stresses through conventional 
      markers of pH, glycolytic potential, and meat quality offered a similar 
      conclusion of the tissue- and stressor-specific responses, validating the kinome 
      results. Collectively, the results of this study highlight the unique cellular 
      responses of breast and thigh tissues to heat and cold stresses and may offer 
      insight into the unique susceptibilities, as well as functional consequences, of 
      these tissues to thermal stress.
CI  - (c) 2015 Poultry Science Association Inc.
FAU - Napper, Scott
AU  - Napper S
AD  - Vaccine and Infectious Disease Organization, University of Saskatchewan, 
      Saskatoon, Saskatchewan S7N 5E3, Canada Department of Biochemistry, University of 
      Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada scott.napper@usask.ca.
FAU - Dadgar, Samira
AU  - Dadgar S
AD  - Department of Food and Bioproduct Science, University of Saskatchewan, Saskatoon, 
      Saskatchewan S7N 5A8, Canada.
FAU - Arsenault, Ryan J
AU  - Arsenault RJ
AD  - United States Department of Agriculture, Agricultural Research Service, SPARC, 
      College Station, TX 77845 USA.
FAU - Trost, Brett
AU  - Trost B
AD  - Department of Computer Science, University of Saskatchewan, Saskatoon, 
      Saskatchewan S7N 5C9, Canada.
FAU - Scruten, Erin
AU  - Scruten E
AD  - Vaccine and Infectious Disease Organization, University of Saskatchewan, 
      Saskatoon, Saskatchewan S7N 5E3, Canada.
FAU - Kusalik, Anthony
AU  - Kusalik A
AD  - Department of Computer Science, University of Saskatchewan, Saskatoon, 
      Saskatchewan S7N 5C9, Canada.
FAU - Shand, Phyllis
AU  - Shand P
AD  - Department of Food and Bioproduct Science, University of Saskatchewan, Saskatoon, 
      Saskatchewan S7N 5A8, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150401
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Avian Proteins)
RN  - 0 (Proteome)
RN  - EC 2.7.- (Phosphotransferases)
SB  - IM
MH  - Animals
MH  - Avian Proteins/*genetics/metabolism
MH  - Chickens/genetics/*physiology
MH  - *Cold-Shock Response
MH  - *Heat-Shock Response
MH  - Male
MH  - Muscle, Skeletal/metabolism
MH  - Organ Specificity
MH  - Pectoralis Muscles/metabolism
MH  - Phosphotransferases/*genetics/metabolism
MH  - Proteome/*genetics/metabolism
OTO - NOTNLM
OT  - chickens
OT  - kinome
OT  - peptide array
OT  - thermal stress
EDAT- 2015/04/04 06:00
MHDA- 2015/08/26 06:00
CRDT- 2015/04/04 06:00
PHST- 2014/12/10 00:00 [accepted]
PHST- 2015/04/04 06:00 [entrez]
PHST- 2015/04/04 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
AID - S0032-5791(19)31926-1 [pii]
AID - 10.3382/ps/pev046 [doi]
PST - ppublish
SO  - Poult Sci. 2015 Jun;94(6):1333-45. doi: 10.3382/ps/pev046. Epub 2015 Apr 1.