PMID- 25841799
OWN - NLM
STAT- MEDLINE
DCOM- 20160208
LR  - 20161020
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 17
IP  - 8
DP  - 2015 Aug
TI  - The response of Paracoccidioides spp. to nitrosative stress.
PG  - 575-85
LID - S1286-4579(15)00064-7 [pii]
LID - 10.1016/j.micinf.2015.03.012 [doi]
AB  - Paracoccidioidomycosis (PCM) is an endemic disease in Latin America caused by 
      species belonging to the genus Paracoccidioides. During infection, immune cells 
      present a variety of defense mechanisms against pathogens. One of these defensive 
      strategies is the production and release of nitric oxide (NO) and S-nitroso 
      thiols (e.g., S-nitrosoglutathione, GSNO), which produce reactive nitrogen 
      species (RNS). This results in damage to DNA and membranes, inhibition of 
      respiration and inactivation of cellular enzymes. In response to nitrosative 
      stress, human pathogenic fungi possess defense mechanisms to prevent the adverse 
      effects of NO, which helps them survive during initial contact with the host 
      immune system. To understand how Paracoccidioides spp. respond to nitrosative 
      stress, we conducted this study to identify genes and proteins that might 
      contribute to this response. The results of proteomic analysis demonstrated that 
      nitrosative stress induced a reduction in the expression of proteins related to 
      the mitochondrial electron transport chain. This hypothesis was supported by the 
      reduced mitochondrial activity observed in the presence of GSNO. Additionally, 
      lipids and branched chain amino acid metabolism enzymes were altered. The role 
      played by enzymes acting in oxidative stress in the RNS response was remarkable. 
      This interface among enzymes acting in both stress responses was confirmed by 
      using a RNA approach to silence the ccp gene in Paracoccidioides. It was observed 
      that mutants with low expression of the ccp gene were more sensitive to 
      nitrosative stress.
CI  - Copyright (c) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights 
      reserved.
FAU - Parente, Ana F A
AU  - Parente AF
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Campus Samambaia, Instituto de Ciencias Biologicas II (ICB II), 
      2 masculine Andar, Sala 206, Goiania, Goias, 74690-900, Brazil; Departamento de Ciencias 
      Fisiologicas, Universidade Federal do Amazonas, Av. General Rodrigo Octavio, 
      6200, Coroado I, Manaus, Amazonas, 69077-000, Brazil.
FAU - Naves, Priscila E C
AU  - Naves PE
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Campus Samambaia, Instituto de Ciencias Biologicas II (ICB II), 
      2 masculine Andar, Sala 206, Goiania, Goias, 74690-900, Brazil.
FAU - Pigosso, Laurine L
AU  - Pigosso LL
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Campus Samambaia, Instituto de Ciencias Biologicas II (ICB II), 
      2 masculine Andar, Sala 206, Goiania, Goias, 74690-900, Brazil.
FAU - Casaletti, Luciana
AU  - Casaletti L
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Campus Samambaia, Instituto de Ciencias Biologicas II (ICB II), 
      2 masculine Andar, Sala 206, Goiania, Goias, 74690-900, Brazil.
FAU - McEwen, Juan G
AU  - McEwen JG
AD  - Unidad de Biologia Celular y Molecular, Corporacion para Investigaciones 
      Biologicas (CIB), Medellin, Calle 62 No. 52-59, 51922, Colombia; Facultad de 
      Medicina, Universidad de Antioquia, Medellin, Carrera 51 D No. 62-29, 050010, 
      Colombia.
FAU - Parente-Rocha, Juliana A
AU  - Parente-Rocha JA
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Campus Samambaia, Instituto de Ciencias Biologicas II (ICB II), 
      2 masculine Andar, Sala 206, Goiania, Goias, 74690-900, Brazil.
FAU - Soares, Celia M A
AU  - Soares CM
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Campus Samambaia, Instituto de Ciencias Biologicas II (ICB II), 
      2 masculine Andar, Sala 206, Goiania, Goias, 74690-900, Brazil. Electronic address: 
      cmasoares@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150402
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Fungal Proteins)
RN  - 0 (Reactive Nitrogen Species)
SB  - IM
MH  - Fungal Proteins/genetics
MH  - Humans
MH  - Nitrosation/immunology
MH  - Oxidative Stress/genetics/*physiology
MH  - Paracoccidioides/genetics/*physiology
MH  - Paracoccidioidomycosis/microbiology
MH  - Reactive Nitrogen Species/immunology/*metabolism
OTO - NOTNLM
OT  - Cytochrome c peroxidase
OT  - Functional analysis
OT  - Paracoccidioides spp.
OT  - Proteomic
OT  - Reactive nitrogen species
EDAT- 2015/04/07 06:00
MHDA- 2016/02/09 06:00
CRDT- 2015/04/06 06:00
PHST- 2015/01/07 00:00 [received]
PHST- 2015/03/05 00:00 [revised]
PHST- 2015/03/23 00:00 [accepted]
PHST- 2015/04/06 06:00 [entrez]
PHST- 2015/04/07 06:00 [pubmed]
PHST- 2016/02/09 06:00 [medline]
AID - S1286-4579(15)00064-7 [pii]
AID - 10.1016/j.micinf.2015.03.012 [doi]
PST - ppublish
SO  - Microbes Infect. 2015 Aug;17(8):575-85. doi: 10.1016/j.micinf.2015.03.012. Epub 
      2015 Apr 2.