PMID- 25845229
OWN - NLM
STAT- MEDLINE
DCOM- 20150417
LR  - 20150407
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 48
IP  - 6
DP  - 2014 Nov-Dec
TI  - [Fluorescence in situ hybridization with DNA probes derived from individual 
      chromosomes and chromosome regions].
PG  - 881-90
AB  - A significant part of the eukaryotic genomes consists of repetitive DNA, which 
      can form large clusters or distributed along euchromatic chromosome regions. 
      Repeats located in chromosomal regions make a problem in analysis and 
      identification of the chromosomal material with fluorescence in situ 
      hybridization (FISH). In most cases, the identification of chromosome regions 
      using FISH requires detection of the signal produced with unique sequences. The 
      feasibility, advantages and disadvantages of traditional methods of suppression 
      of repetitive DNA hybridization, methods of repeats-free probe construction and 
      methods of chromosome-specific DNA sequences visualization using image processing 
      of multicolor FISH results are considered in the paper. The efficiency of 
      different techniques for DNA probe generation, different FISH protocols, and 
      image processing of obtained microscopic images depends on the genomic size and 
      structure of analyzing species. This problem was discussed and different 
      approaches were considered for the analysis of the species with very large 
      genome, rare species and species which specimens are too small in size to obtain 
      the amount of genomic and Cot-1 DNA required for suppression of repetitive DNA 
      hybridization.
FAU - Bogomolov, A G
AU  - Bogomolov AG
FAU - Karamysheva, T V
AU  - Karamysheva TV
FAU - Rubtsov, N B
AU  - Rubtsov NB
LA  - rus
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (DNA Probes)
RN  - 0 (Euchromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - *Artifacts
MH  - Chromosomes/*chemistry/ultrastructure
MH  - DNA/analysis/genetics
MH  - DNA Probes/*chemistry/metabolism
MH  - Euchromatin/*chemistry/ultrastructure
MH  - Eukaryotic Cells/metabolism/ultrastructure
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/instrumentation/*standards
MH  - Metaphase
MH  - Microscopy, Fluorescence/standards
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2015/04/08 06:00
MHDA- 2015/04/18 06:00
CRDT- 2015/04/08 06:00
PHST- 2015/04/08 06:00 [entrez]
PHST- 2015/04/08 06:00 [pubmed]
PHST- 2015/04/18 06:00 [medline]
PST - ppublish
SO  - Mol Biol (Mosk). 2014 Nov-Dec;48(6):881-90.