PMID- 25847252
OWN - NLM
STAT- MEDLINE
DCOM- 20160613
LR  - 20150903
IS  - 1600-0781 (Electronic)
IS  - 0905-4383 (Linking)
VI  - 31
IP  - 5
DP  - 2015 Sep
TI  - Ex vivo evaluation of the effect of photodynamic therapy on skin scars and striae 
      distensae.
PG  - 239-51
LID - 10.1111/phpp.12180 [doi]
AB  - BACKGROUND: Skin scars and striae distensae (SD) are common dermal disorders with 
      ill-defined treatment options. There is emerging clinical evidence for use of 
      photodynamic therapy (PDT) in treating skin fibrosis. Therefore, the aim here was 
      to investigate the effect of PDT on skin scars and SD in an ex vivo model of 
      human skin scarring. METHODS: Photodynamic therapy, with 5ALA or MALA in addition 
      to illumination with 40 J/cm(2) of red light, was applied to striae alba, fine 
      line, hypertrophic and keloid scars ex vivo (n = 18). General morphology was 
      assessed by H&E, Herovici's and Weigert's differential staining. Apoptosis, 
      proliferation, metalloproteinase 3 and tropoelastin expression were quantified 
      immunohistochemically, and differential gene expression of proliferating cell 
      nuclear antigen (PCNA), collagen (COL) type I and type III, matrix 
      metalloproteinase 3 (MMP3) and tropoelastin (ELN) was assessed by real-time 
      quantitative reverse transcription polymerase chain reaction. RESULTS: Apoptosis 
      increased, which correlated with decreased proliferation and PCNA gene 
      expression. Post-PDT, matrix components were found to be re-organised in both 
      hypertrophic and keloid scars. COLI and COLIII gene expression levels decreased, 
      whilst MMP3 and ELN increased significantly post-PDT compared to normal skin and 
      untreated controls (P < 0.05). However, no significant difference between 5ALA 
      and MALA-PDT treatments was observed. CONCLUSION: Using our unique ex vivo model, 
      we show for the first time morphological and cellular effect of application of 
      PDT, which correlates with the degree and severity of dermal fibrosis. In view of 
      this, PDT may be ideal in targeting treatment of abnormal skin scarring.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Mendoza-Garcia, Jenifer
AU  - Mendoza-Garcia J
AD  - Bioengineering Group, School of Materials, The University of Manchester, 
      Manchester, UK.
AD  - Plastic & Reconstructive Surgery Research Group, Manchester Institute of 
      Biotechnology (MIB), The University of Manchester, Manchester, UK.
FAU - Sebastian, Anil
AU  - Sebastian A
AD  - Plastic & Reconstructive Surgery Research Group, Manchester Institute of 
      Biotechnology (MIB), The University of Manchester, Manchester, UK.
FAU - Alonso-Rasgado, Teresa
AU  - Alonso-Rasgado T
AD  - Bioengineering Group, School of Materials, The University of Manchester, 
      Manchester, UK.
FAU - Bayat, Ardeshir
AU  - Bayat A
AD  - Bioengineering Group, School of Materials, The University of Manchester, 
      Manchester, UK.
AD  - Plastic & Reconstructive Surgery Research Group, Manchester Institute of 
      Biotechnology (MIB), The University of Manchester, Manchester, UK.
AD  - University Hospital of South Manchester NHS Foundation Trust, Centre for 
      Dermatology, Institute of Inflammation and Repair, Faculty of Medical and Human 
      Sciences, University of Manchester, Manchester Academic Health Science Centre, 
      Manchester, UK.
LA  - eng
PT  - Journal Article
DEP - 20150601
PL  - England
TA  - Photodermatol Photoimmunol Photomed
JT  - Photodermatology, photoimmunology & photomedicine
JID - 9013641
RN  - 0 (Photosensitizing Agents)
RN  - 88755TAZ87 (Aminolevulinic Acid)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aminolevulinic Acid/therapeutic use
MH  - Cicatrix, Hypertrophic/*drug therapy
MH  - Female
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Middle Aged
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/therapeutic use
MH  - Skin/enzymology
MH  - Striae Distensae/*drug therapy
OTO - NOTNLM
OT  - ex vivo
OT  - keloid and hypertrophic scar
OT  - photodynamic therapy
OT  - scar models
OT  - striae distensae
EDAT- 2015/04/08 06:00
MHDA- 2016/06/14 06:00
CRDT- 2015/04/08 06:00
PHST- 2015/03/30 00:00 [accepted]
PHST- 2015/04/08 06:00 [entrez]
PHST- 2015/04/08 06:00 [pubmed]
PHST- 2016/06/14 06:00 [medline]
AID - 10.1111/phpp.12180 [doi]
PST - ppublish
SO  - Photodermatol Photoimmunol Photomed. 2015 Sep;31(5):239-51. doi: 
      10.1111/phpp.12180. Epub 2015 Jun 1.