PMID- 25847260
OWN - NLM
STAT- MEDLINE
DCOM- 20160316
LR  - 20150611
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 2
DP  - 2015 Aug
TI  - Inhibition of expression of hepatitis C virus 1b genotype core and NS4B genes in 
      HepG2 cells using artificial microRNAs.
PG  - 1905-13
LID - 10.3892/mmr.2015.3571 [doi]
AB  - The present study aimed to evaluate the silencing effect of artificial microRNAs 
      (amiRNAs) against the hepatitis C virus (HCV) 1b (HCV1b) genotype core (C) and 
      non-structural protein 4B (NS4B) genes. pDsRed-monomer-Core and 
      pDsRed-monomer-NS4B plasmids, containing the target genes were constructed. A 
      total of eight artificial micro RNA (amiRNA)-expressing plasmids, namely, 
      pmiRE-C-mi1 to -mi4 and pmiRE-NS4B-mi1 to -mi4, were designed and constructed to 
      interfere with various sites of the core and NS4B genes, and the amiRNA 
      interfering plasmid and the corresponding target gene-expressing plasmid were 
      co-transfected into HepG2 cells. At 48 h after transfection, HCV core and NS4B 
      gene expression levels were detected using fluorescence microscopy, flow 
      cytometry, reverse transcription quantitative polymerase chain reaction and 
      western blot analysis. Fluorescence microscopy revealed that the target 
      gene-transfected cells expressed red fluorescent protein, whereas the interfering 
      plasmid-transfected cells exhibited expression of green fluorescent protein. The 
      percentage of red fluorescent proteins and mean fluorescence intensity, as well 
      as protein expression levels of the core and NS4B genes within the cells, which 
      were co-transfected by the amiRNA interfering plasmid and the target gene, were 
      significantly decreased. The results of the present study confirmed that amiRNAs 
      may effectively and specifically inhibit the expression of HCV1b core and NS4B 
      genes in HepG2 cells, potentially providing a novel therapeutic strategy for the 
      treatment of HCV.
FAU - Jiang, Xiao-Hua
AU  - Jiang XH
AD  - Department of Infectious Diseases, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410087, P.R. China.
FAU - Xie, Yu-Tao
AU  - Xie YT
AD  - Department of Infectious Diseases, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410087, P.R. China.
FAU - Jiang, Bo
AU  - Jiang B
AD  - Department of Infectious Diseases, The First Affiliated Hospital of The 
      University of South China, Hengyang, Hunan 421001, P.R. China.
FAU - Tang, Meng-Ying
AU  - Tang MY
AD  - Department of Infectious Diseases, The First Affiliated Hospital of The 
      University of South China, Hengyang, Hunan 421001, P.R. China.
FAU - Ma, Tao
AU  - Ma T
AD  - Department of Infectious Diseases, The First Affiliated Hospital of The 
      University of South China, Hengyang, Hunan 421001, P.R. China.
FAU - Peng, Hua
AU  - Peng H
AD  - Department of Infectious Diseases, The First Affiliated Hospital of The 
      University of South China, Hengyang, Hunan 421001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20150331
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (NS4B protein, flavivirus)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Viral Core Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Female
MH  - Genotype
MH  - Hep G2 Cells
MH  - Hepacivirus/*genetics/isolation & purification/metabolism
MH  - Hepatitis C/metabolism/pathology/virology
MH  - Humans
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Transfection
MH  - Viral Core Proteins/*antagonists & inhibitors/genetics/metabolism
MH  - Viral Nonstructural Proteins/*antagonists & inhibitors/genetics/metabolism
EDAT- 2015/04/08 06:00
MHDA- 2016/03/17 06:00
CRDT- 2015/04/08 06:00
PHST- 2014/03/28 00:00 [received]
PHST- 2015/02/05 00:00 [accepted]
PHST- 2015/04/08 06:00 [entrez]
PHST- 2015/04/08 06:00 [pubmed]
PHST- 2016/03/17 06:00 [medline]
AID - 10.3892/mmr.2015.3571 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Aug;12(2):1905-13. doi: 10.3892/mmr.2015.3571. Epub 2015 Mar 
      31.