PMID- 25848249
OWN - NLM
STAT- MEDLINE
DCOM- 20161028
LR  - 20181202
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 10
DP  - 2015
TI  - Silica nanoparticles increase human adipose tissue-derived stem cell 
      proliferation through ERK1/2 activation.
PG  - 2261-72
LID - 10.2147/IJN.S71925 [doi]
AB  - BACKGROUND: Silicon dioxide composites have been found to enhance the mechanical 
      properties of scaffolds and to support growth of human adipose tissue-derived 
      stem cells (hADSCs) both in vitro and in vivo. Silica (silicon dioxide alone) 
      exists as differently sized particles when suspended in culture medium, but it is 
      not clear whether particle size influences the beneficial effect of silicon 
      dioxide on hADSCs. In this study, we examined the effect of different sized 
      particles on growth and mitogen-activated protein kinase signaling in hADSCs. 
      METHODS: Silica gel was prepared by a chemical reaction using hydrochloric acid 
      and sodium silicate, washed, sterilized, and suspended in serum-free culture 
      medium for 48 hours, and then sequentially filtered through a 0.22 mum filter 
      (filtrate containing nanoparticles smaller than 220 nm; silica NPs). hADSCs were 
      incubated with silica NPs or 3 mum silica microparticles (MPs), examined by 
      transmission electron microscopy, and assayed for cell proliferation, apoptosis, 
      and mitogen-activated protein kinase signaling. RESULTS: Eighty-nine percent of 
      the silica NPs were around 50-120 nm in size. When hADSCs were treated with the 
      study particles, silica NPs were observed in endocytosed vacuoles in the cytosol 
      of hADSCs, but silica MPs showed no cell entry. Silica NPs increased the 
      proliferation of hADSCs, but silica MPs had no significant effect in this regard. 
      Instead, silica MPs induced slight apoptosis. Silica NPs increased 
      phosphorylation of extracellular signal-related kinase (ERK)1/2, while silica MPs 
      increased phosphorylation of p38. Silica NPs had no effect on phosphorylation of 
      Janus kinase or p38. Pretreatment with PD98059, a MEK inhibitor, prevented the 
      ERK1/2 phosphorylation and proliferation induced by silica NPs. CONCLUSION: 
      Scaffolds containing silicon dioxide for tissue engineering may enhance cell 
      growth through ERK1/2 activation only when NPs around 50-120 nm in size are 
      included, and single component silica-derived NPs could be useful for 
      bioscaffolds in stem cell therapy.
FAU - Kim, Ki Joo
AU  - Kim KJ
AD  - Department of Plastic Surgery, College of Medicine, The Catholic University of 
      Korea, Seoul, Republic of Korea ; Department of Molecular Biomedicine, The 
      Catholic University of Korea, Seoul, Republic of Korea.
FAU - Joe, Young Ae
AU  - Joe YA
AD  - Cancer Research Institute and Department of Medical Lifescience, The Catholic 
      University of Korea, Seoul, Republic of Korea.
FAU - Kim, Min Kyoung
AU  - Kim MK
AD  - Department of Plastic Surgery, College of Medicine, The Catholic University of 
      Korea, Seoul, Republic of Korea ; Department of Molecular Biomedicine, The 
      Catholic University of Korea, Seoul, Republic of Korea.
FAU - Lee, Su Jin
AU  - Lee SJ
AD  - Department of Plastic Surgery, College of Medicine, The Catholic University of 
      Korea, Seoul, Republic of Korea.
FAU - Ryu, Yeon Hee
AU  - Ryu YH
AD  - Department of Plastic Surgery, College of Medicine, The Catholic University of 
      Korea, Seoul, Republic of Korea ; Department of Molecular Biomedicine, The 
      Catholic University of Korea, Seoul, Republic of Korea.
FAU - Cho, Dong-Woo
AU  - Cho DW
AD  - Department of Mechanical Engineering, Pohang University of Science and 
      Technology, Gyeongbuk, Republic of Korea ; Department of Integrative Bioscience 
      and Bioengineering, Pohang University of Science and Technology, Gyeongbuk, 
      Republic of Korea.
FAU - Rhie, Jong Won
AU  - Rhie JW
AD  - Department of Plastic Surgery, College of Medicine, The Catholic University of 
      Korea, Seoul, Republic of Korea ; Department of Molecular Biomedicine, The 
      Catholic University of Korea, Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150324
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Flavonoids)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 2.7.11.24 (MAPK1 protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Apoptosis/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Flavonoids/pharmacology
MH  - Humans
MH  - MAP Kinase Signaling System
MH  - Microscopy, Electron, Transmission
MH  - Mitogen-Activated Protein Kinase 1/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - *Nanoparticles/chemistry
MH  - Particle Size
MH  - Phosphorylation
MH  - Signal Transduction/drug effects
MH  - Silicon Dioxide/*chemistry/pharmacology
MH  - Stem Cells/*cytology/drug effects/physiology
MH  - Tissue Scaffolds
PMC - PMC4378289
OTO - NOTNLM
OT  - ERK
OT  - biomaterial
OT  - ceramic
OT  - mesenchymal stem cells
OT  - particle
EDAT- 2015/04/08 06:00
MHDA- 2016/11/01 06:00
PMCR- 2015/03/24
CRDT- 2015/04/08 06:00
PHST- 2015/04/08 06:00 [entrez]
PHST- 2015/04/08 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2015/03/24 00:00 [pmc-release]
AID - ijn-10-2261 [pii]
AID - 10.2147/IJN.S71925 [doi]
PST - epublish
SO  - Int J Nanomedicine. 2015 Mar 24;10:2261-72. doi: 10.2147/IJN.S71925. eCollection 
      2015.