PMID- 25849526
OWN - NLM
STAT- MEDLINE
DCOM- 20151020
LR  - 20150507
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 595
DP  - 2015 May 19
TI  - A novel brain-derived neurotrophic factor-modulating peptide attenuates 
      Abeta1-42-induced neurotoxicity in vitro.
PG  - 63-8
LID - S0304-3940(15)00279-7 [pii]
LID - 10.1016/j.neulet.2015.03.070 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family, 
      which plays important roles in learning and memory formation and in protecting 
      neurons from diverse neurotoxic insults, such as amyloid-beta (Abeta). Since BDNF 
      expression is decreased in patients with Alzheimer's disease, various strategies 
      have attempted to increase BDNF levels. In a previous study, we screened and 
      identified a novel BDNF-modulating peptide (consisting of 
      methionine-valine-glycine, named Neuropep-1) by a positional scanning-synthetic 
      peptide combinatorial library (PS-SPCL). Neuropep-1 exhibited neuroprotective 
      effects against in vitro and in vivo Alzheimer's disease models. Based on the 
      previous PS-SPCL data, we modified the amino acid sequence of Neuropep-1 in this 
      study to identify a more potent novel BDNF-modulating peptide. By replacing the 
      valine in the second position with aspartic acid, the resulting Neuropep-4 was 
      found to be highly effective in inducing BDNF expression even at concentrations 
      of 1pM in the SH-SY5Y cell line and rat primary cortical neurons. In addition, 
      among the tested peptides, Neuropep-4 provided neurons with the strongest 
      protection against oligomeric and/or fibrillar Abeta1-42-induced cell death through 
      BDNF upregulation. These results suggest the potential of Neuropep-4 as a 
      therapeutic candidate for treating neurodegenerative diseases, such as AD.
CI  - Copyright (c) 2015. Published by Elsevier Ireland Ltd.
FAU - Shin, Min-Kyoo
AU  - Shin MK
AD  - Department of Biological Sciences, Sungkyunkwan University, 300 Chunchun-Dong, 
      Jangan-Gu, Suwon, Gyeonggi-Do 440-746, South Korea; Institute of Basic Science, 
      Sungkyunkwan University, 300 Chunchun-Dong, Jangan-Gu, Suwon, Gyeonggi-Do 
      440-746, South Korea.
FAU - Kim, Hong-Gi
AU  - Kim HG
AD  - Department of Biological Sciences, Sungkyunkwan University, 300 Chunchun-Dong, 
      Jangan-Gu, Suwon, Gyeonggi-Do 440-746, South Korea.
FAU - Kim, Kil-Lyong
AU  - Kim KL
AD  - Department of Biological Sciences, Sungkyunkwan University, 300 Chunchun-Dong, 
      Jangan-Gu, Suwon, Gyeonggi-Do 440-746, South Korea. Electronic address: 
      kimkl@skku.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150404
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (1-42))
RN  - 0 (methionyl-aspartyl-glycine)
SB  - IM
MH  - Amyloid beta-Peptides/*metabolism/toxicity
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Humans
MH  - Neurons/cytology/drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - Oligopeptides/*pharmacology
MH  - Peptide Fragments/*metabolism/toxicity
MH  - Primary Cell Culture
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Amyloid-beta
OT  - Brain-derived neurotrophic factor
OT  - Neuroprotection
OT  - Peptide
EDAT- 2015/04/08 06:00
MHDA- 2015/10/21 06:00
CRDT- 2015/04/08 06:00
PHST- 2014/09/24 00:00 [received]
PHST- 2015/02/11 00:00 [revised]
PHST- 2015/03/25 00:00 [accepted]
PHST- 2015/04/08 06:00 [entrez]
PHST- 2015/04/08 06:00 [pubmed]
PHST- 2015/10/21 06:00 [medline]
AID - S0304-3940(15)00279-7 [pii]
AID - 10.1016/j.neulet.2015.03.070 [doi]
PST - ppublish
SO  - Neurosci Lett. 2015 May 19;595:63-8. doi: 10.1016/j.neulet.2015.03.070. Epub 2015 
      Apr 4.