PMID- 25850410
OWN - NLM
STAT- MEDLINE
DCOM- 20160322
LR  - 20220409
IS  - 1432-119X (Electronic)
IS  - 0948-6143 (Linking)
VI  - 144
IP  - 1
DP  - 2015 Jul
TI  - Human sperm molecular anatomy: the enzyme 5alpha-reductase (SRD5A) is present in the 
      sperm and may be involved in the varicocele-related infertility.
PG  - 67-76
LID - 10.1007/s00418-015-1320-8 [doi]
AB  - The most common cause of male infertility is the testicular varicocele, a 
      condition that impairs production and decreases quality of sperm. Male fertility 
      also strictly depends on androgens acting through their own receptor. The enzyme 
      5alpha-reductase (SRD5A) is involved in the conversion of testosterone to 
      5alpha-dihydrotestosterone, both required for the development and maintenance of male 
      reproductive function. Here, we evaluated, by western blotting analysis, the 
      presence of SRD5A in human ejaculated spermatozoa and evidenced differences in 
      sperm SRD5A content between healthy donors and varicocele-affected patients. 
      Additionally, SRD5A sperm ultrastructural localization was also assessed by 
      transmission electron microscopy and immunogold assay. We evidenced that SRD5A 
      enzyme is present in the human spermatozoa and that its cellular content is 
      lowered in sperm samples from varicocele patients compared to healthy subjects. 
      The presence of SRD5A in human ejaculated spermatozoa highlights the potential 
      role of this enzyme in sperm physiopathology suggesting that the decrease in its 
      content, by affecting the conversion of testosterone into 5alpha-dihydrotestosterone, 
      may be an important additional mechanism involved in the harmful effect of 
      varicocele in male fertility.
FAU - Aquila, Saveria
AU  - Aquila S
AD  - Centro Sanitario, University of Calabria, 87036, Arcavacata Di Rende, Cosenza, 
      Italy, saveria.aquila@libero.it.
FAU - Montanaro, Daniela
AU  - Montanaro D
FAU - Guido, Carmela
AU  - Guido C
FAU - Santoro, Marta
AU  - Santoro M
FAU - Perrotta, Ida
AU  - Perrotta I
FAU - Gervasi, Serena
AU  - Gervasi S
FAU - De Amicis, Francesca
AU  - De Amicis F
FAU - Lanzino, Marilena
AU  - Lanzino M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150408
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Membrane Proteins)
RN  - 3XMK78S47O (Testosterone)
RN  - EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase)
RN  - EC 1.3.99.5 (SRD5A1 protein, human)
SB  - IM
MH  - 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/*metabolism
MH  - Adult
MH  - Humans
MH  - Immunohistochemistry
MH  - Infertility/*etiology
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Immunoelectron
MH  - Spermatozoa/*enzymology/physiology/ultrastructure
MH  - Testosterone/metabolism
MH  - Varicocele/*enzymology/physiopathology
EDAT- 2015/04/09 06:00
MHDA- 2016/03/24 06:00
CRDT- 2015/04/09 06:00
PHST- 2015/03/28 00:00 [accepted]
PHST- 2015/04/09 06:00 [entrez]
PHST- 2015/04/09 06:00 [pubmed]
PHST- 2016/03/24 06:00 [medline]
AID - 10.1007/s00418-015-1320-8 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2015 Jul;144(1):67-76. doi: 10.1007/s00418-015-1320-8. Epub 
      2015 Apr 8.