PMID- 25851410
OWN - NLM
STAT- MEDLINE
DCOM- 20160512
LR  - 20210410
IS  - 1573-904X (Electronic)
IS  - 0724-8741 (Print)
IS  - 0724-8741 (Linking)
VI  - 32
IP  - 9
DP  - 2015 Sep
TI  - Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s.
PG  - 3066-86
LID - 10.1007/s11095-015-1688-0 [doi]
AB  - PURPOSE: To investigate the properties of phenylboronic acid-functional 
      poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with 
      poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their 
      compatibility with COS-7 cells. METHODS: Copolymers of phenylboronic 
      acid-functional poly(amido amine)s, differing in the content of primary amine 
      (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied 
      in the formation of multilayers with PVA and ChS. Biocompatibility of the 
      resulting films was evaluated through cell culture experiments with COS-7 cells 
      grown on the films. RESULTS: PVA-based multilayers were thin, reaching ~100 nm at 
      10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the 
      same number of bilayers. All of the multilayers are stable under physiological 
      conditions in vitro and are responsive to reducing agents, owing to the presence 
      of disulfide bonds in the polymers. PVA-based films were demonstrated to be 
      responsive to glucose at physiological pH at the investigated glucose 
      concentrations (10-100 mM). The multilayered films displayed biocompatibility in 
      cell culture experiments, promoting attachment and proliferation of COS-7 cells. 
      CONCLUSIONS: Responsive thin films based on boronic acid functional poly(amido 
      amine)s are promising biocompatible materials for biomedical applications, such 
      as drug releasing surfaces on stents or implants. Graphical Abstract 
      Layer-by-Layer Assembly.
FAU - Hujaya, Sry D
AU  - Hujaya SD
AD  - Department of Controlled Drug Delivery, MIRA Institute for Biomedical Technology 
      and Technical Medicine, Faculty of Science and Technology, University of Twente, 
      P.O. Box 217, 7500 AE, Enschede, The Netherlands.
FAU - Engbersen, Johan F J
AU  - Engbersen JF
FAU - Paulusse, Jos M J
AU  - Paulusse JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150408
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (Biocompatible Materials)
RN  - 0 (Boronic Acids)
RN  - 0 (Poly(amidoamine))
RN  - 0 (Polyamines)
RN  - 0 (Polymers)
RN  - 9002-89-5 (Polyvinyl Alcohol)
RN  - 9007-28-7 (Chondroitin Sulfates)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Boronic Acids/chemistry
MH  - COS Cells
MH  - Cell Proliferation/drug effects
MH  - Chlorocebus aethiops
MH  - Chondroitin Sulfates/chemistry
MH  - Glucose/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Polyamines/*chemistry
MH  - Polymers/*chemistry
MH  - Polyvinyl Alcohol/chemistry
PMC - PMC4526598
EDAT- 2015/04/09 06:00
MHDA- 2016/05/14 06:00
PMCR- 2015/04/08
CRDT- 2015/04/09 06:00
PHST- 2015/02/23 00:00 [received]
PHST- 2015/03/23 00:00 [accepted]
PHST- 2015/04/09 06:00 [entrez]
PHST- 2015/04/09 06:00 [pubmed]
PHST- 2016/05/14 06:00 [medline]
PHST- 2015/04/08 00:00 [pmc-release]
AID - 1688 [pii]
AID - 10.1007/s11095-015-1688-0 [doi]
PST - ppublish
SO  - Pharm Res. 2015 Sep;32(9):3066-86. doi: 10.1007/s11095-015-1688-0. Epub 2015 Apr 
      8.