PMID- 25851919
OWN - NLM
STAT- MEDLINE
DCOM- 20160118
LR  - 20150423
IS  - 1601-183X (Electronic)
IS  - 1601-183X (Linking)
VI  - 14
IP  - 4
DP  - 2015 Apr
TI  - Strain-dependent variations in visceral sensitivity: relationship to stress, 
      anxiety and spinal glutamate transporter expression.
PG  - 319-29
LID - 10.1111/gbb.12216 [doi]
AB  - Responses to painful stimuli differ between populations, ethnic groups, sexes and 
      even among individuals of a family. However, data regarding visceral pain are 
      still lacking. Thus, we investigated differences in visceral nociception across 
      inbred and outbred mouse strains using colorectal distension. Anxiety and 
      depression-like behaviour were assessed using the open field and forced swim test 
      as well as the corticosterone stress response. Possible mechanistic targets 
      [excitatory amino acid transporter (EAAT-1), brain-derived neurotrophic factor 
      (BDNF) and 5HT1A receptor] were also assessed using quantitative real-time 
      polymerase chain reaction. Adult, male, inbred and outbred mouse strains were 
      used in all assays (inbred strains; CBA/J Hsd, C3H/HeNHsd, BALB/c OlaHsd, C57 
      BL/6JOlaHsd, DBA/2J RccHsd, CAST/EiJ, SM/J, A/J OlaHsd, 129P2/OlaHsd, FVB/NHan 
      Hsd and outbred strains: Swiss Webster, CD-1). mRNA expression levels of EAAT-1, 
      BDNF and 5HT1A receptor (HTR1A) were quantified in the lumbosacral spinal cord, 
      amygdala and hippocampus. A significant effect of strain was found in visceral 
      sensitivity, anxiety and depressive-like behaviours. Strain differences were also 
      seen in both baseline and stress-induced corticosterone levels. CBA/J mice 
      consistently exhibited heightened visceral sensitivity, anxiety behaviour and 
      depression-like behaviour which were associated with decreased spinal EAAT-1 and 
      hippocampal BDNF and HTR1A. Our results show the CBA/J mouse strain as a novel 
      mouse model to unravel the complex mechanisms of brain-gut axis disorders such as 
      irritable bowel syndrome, in particular the underlying mechanisms of visceral 
      hypersensitivity, for which there is great need. Furthermore, this study 
      highlights the importance of genotype and the consequences for future development 
      of transgenic strains in pain research.
CI  - (c) 2015 John Wiley & Sons Ltd and International Behavioural and Neural Genetics 
      Society.
FAU - Moloney, R D
AU  - Moloney RD
AD  - Laboratory of Neurogastroenterology, Alimentary Pharmabiotic Centre, Biosciences 
      Institute, Ireland; Department of Psychiatry, Ireland.
FAU - Dinan, T G
AU  - Dinan TG
FAU - Cryan, J F
AU  - Cryan JF
LA  - eng
PT  - Journal Article
DEP - 20150421
PL  - England
TA  - Genes Brain Behav
JT  - Genes, brain, and behavior
JID - 101129617
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Excitatory Amino Acid Transporter 1)
RN  - 0 (Slc1a3 protein, mouse)
RN  - 112692-38-3 (Receptor, Serotonin, 5-HT1A)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Amino Acid Transport System X-AG/genetics/*metabolism
MH  - Amygdala/metabolism
MH  - Animals
MH  - Anxiety/*genetics/physiopathology
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Corticosterone/blood
MH  - Excitatory Amino Acid Transporter 1/genetics/*metabolism
MH  - Hippocampus/metabolism
MH  - Intestines/*innervation
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - *Nociception
MH  - Receptor, Serotonin, 5-HT1A/genetics/metabolism
MH  - Spinal Cord/*metabolism
MH  - Stress, Psychological/*genetics/physiopathology
OTO - NOTNLM
OT  - BDNF
OT  - animal behaviour
OT  - anxiety
OT  - colorectal distension
OT  - depression
OT  - glutamate
OT  - mouse strain
OT  - serotonin
OT  - stress
OT  - visceral pain
EDAT- 2015/04/09 06:00
MHDA- 2016/01/19 06:00
CRDT- 2015/04/09 06:00
PHST- 2015/04/09 06:00 [entrez]
PHST- 2015/04/09 06:00 [pubmed]
PHST- 2016/01/19 06:00 [medline]
AID - 10.1111/gbb.12216 [doi]
PST - ppublish
SO  - Genes Brain Behav. 2015 Apr;14(4):319-29. doi: 10.1111/gbb.12216. Epub 2015 Apr 
      21.