PMID- 25852486
OWN - NLM
STAT- MEDLINE
DCOM- 20151105
LR  - 20181113
IS  - 1662-5110 (Electronic)
IS  - 1662-5110 (Linking)
VI  - 9
DP  - 2015
TI  - Glycine and GABAA receptors mediate tonic and phasic inhibitory processes that 
      contribute to prepulse inhibition in the goldfish startle network.
PG  - 12
LID - 10.3389/fncir.2015.00012 [doi]
LID - 12
AB  - Prepulse inhibition (PPI) is understood as a sensorimotor gating process that 
      attenuates sensory flow to the startle pathway during early stages (20-1000 ms) 
      of information processing. Here, we applied in vivo electrophysiology and 
      pharmacology to determine if PPI is mediated by glycine receptors (GlyRs) and/or 
      GABAA receptors (GABAARs) in the goldfish auditory startle circuit. Specifically, 
      we used selective antagonists to dissect the contributions of target receptors on 
      sound-evoked postsynaptic potentials (PSPs) recorded in the neurons that initiate 
      startle, the Mauthner-cells (M-cell). We found that strychnine, a GlyR 
      antagonist, disrupted a fast-activated (5 ms) and rapidly (<50 ms) decaying 
      (feed-forward) inhibitory process that contributes to PPI at 20 ms prepulse/pulse 
      inter-stimulus intervals (ISI). Additionally we observed increases of the evoked 
      postsynaptic potential (PSP) peak amplitude (+87.43 +/- 21.53%, N = 9) and duration 
      (+204 +/- 48.91%, N = 9). In contrast, treatment with bicuculline, a GABAAR 
      antagonist, caused a general reduction in PPI across all tested interstimulus 
      intervals (ISIs) (20-500 ms). Bicuculline also increased PSP peak amplitude 
      (+133.8 +/- 10.3%, N = 5) and PSP duration (+284.95 +/- 65.64%, N = 5). Treatment 
      with either antagonist also tonically increased post-synaptic excitability in the 
      M-cells, reflected by an increase in the magnitude of antidromically-evoked 
      action potentials (APs) by 15.07 +/- 3.21%, N = 7 and 16.23 +/- 7.08%, N = 5 for 
      strychnine and bicuculline, respectively. These results suggest that GABAARs and 
      GlyRs are functionally segregated to short- and longer-lasting sound-evoked 
      (phasic) inhibitory processes that contribute to PPI, with the mediation of tonic 
      inhibition by both receptor systems being critical for gain control within the 
      M-cell startle circuit.
FAU - Curtin, Paul C P
AU  - Curtin PC
AD  - Graduate Center, City University of New York New York, NY, USA.
FAU - Preuss, Thomas
AU  - Preuss T
AD  - Hunter College, City University of New York New York, NY, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150324
PL  - Switzerland
TA  - Front Neural Circuits
JT  - Frontiers in neural circuits
JID - 101477940
RN  - 0 (Receptors, GABA-A)
RN  - 0 (Receptors, Glycine)
SB  - IM
MH  - Acoustic Stimulation
MH  - Animals
MH  - Goldfish
MH  - Neural Inhibition/*physiology
MH  - Patch-Clamp Techniques
MH  - Prepulse Inhibition/*physiology
MH  - Receptors, GABA-A/*metabolism
MH  - Receptors, Glycine/*metabolism
MH  - Reflex, Startle/*physiology
PMC - PMC4371714
OTO - NOTNLM
OT  - Mauthner cell
OT  - auditory startle circuit
OT  - phasic inhibition
OT  - prepulse inhibition
OT  - sensorimotor integration
OT  - sensory processing
OT  - tonic inhibition
EDAT- 2015/04/09 06:00
MHDA- 2015/11/06 06:00
PMCR- 2015/01/01
CRDT- 2015/04/09 06:00
PHST- 2014/11/16 00:00 [received]
PHST- 2015/03/04 00:00 [accepted]
PHST- 2015/04/09 06:00 [entrez]
PHST- 2015/04/09 06:00 [pubmed]
PHST- 2015/11/06 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fncir.2015.00012 [doi]
PST - epublish
SO  - Front Neural Circuits. 2015 Mar 24;9:12. doi: 10.3389/fncir.2015.00012. 
      eCollection 2015.