PMID- 25854597
OWN - NLM
STAT- MEDLINE
DCOM- 20160525
LR  - 20181202
IS  - 1552-4957 (Electronic)
IS  - 1552-4949 (Linking)
VI  - 88
IP  - 5
DP  - 2015 Sep-Oct
TI  - Optimization of EGFR high positive cell isolation procedure by design of 
      experiments methodology.
PG  - 338-47
LID - 10.1002/cyto.b.21246 [doi]
AB  - BACKGROUND: Circulating tumor cells (CTCs) in blood circulation may play a role 
      in monitoring and even in early detection of metastasis patients. Due to the 
      limited presence of CTCs in blood circulation, viable CTCs isolation technology 
      must supply a very high recovery rate. METHODS: Here, we implement design of 
      experiments (DOE) methodology in order to optimize the Bio-Ferrography (BF) 
      immunomagnetic isolation (IMI) procedure for the EGFR high positive CTCs 
      application. All consequent DOE phases such as screening design, optimization 
      experiments and validation experiments were used. RESULTS: A significant recovery 
      rate of more than 95% was achieved while isolating 100 EGFR high positive CTCs 
      from 1 mL human whole blood. CONCLUSIONS: The recovery achievement in this 
      research positions BF technology as one of the most efficient IMI technologies, 
      which is ready to be challenged with patients' blood samples.
CI  - (c) 2015 International Clinical Cytometry Society.
FAU - Levi, Ofer
AU  - Levi O
AD  - Biomaterials and Corrosion Lab, Department of Materials Science and Engineering, 
      Tel-Aviv University, Ramat Aviv 6997801, Israel.
FAU - Tal, Baruch
AU  - Tal B
AD  - Biomaterials and Corrosion Lab, Department of Materials Science and Engineering, 
      Tel-Aviv University, Ramat Aviv 6997801, Israel.
FAU - Hileli, Sagi
AU  - Hileli S
AD  - Advanced Logistics Development Ltd, Tel Aviv 6713701, Israel.
FAU - Shapira, Assaf
AU  - Shapira A
AD  - Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, 
      Ramat Aviv, Tel-Aviv 6997801, Israel.
FAU - Benhar, Itai
AU  - Benhar I
AD  - Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, 
      Ramat Aviv, Tel-Aviv 6997801, Israel.
FAU - Grabov, Pavel
AU  - Grabov P
AD  - Advanced Logistics Development Ltd, Tel Aviv 6713701, Israel.
FAU - Eliaz, Noam
AU  - Eliaz N
AD  - Biomaterials and Corrosion Lab, Department of Materials Science and Engineering, 
      Tel-Aviv University, Ramat Aviv 6997801, Israel.
LA  - eng
PT  - Journal Article
DEP - 20150418
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Cell Line, Tumor
MH  - Equipment Design
MH  - ErbB Receptors/*analysis
MH  - Flow Cytometry/instrumentation/*methods
MH  - Humans
MH  - Immunomagnetic Separation/instrumentation/*methods
MH  - Neoplastic Cells, Circulating/*chemistry
OTO - NOTNLM
OT  - EGFR
OT  - bio-ferrography
OT  - carcinoma
OT  - design of experiments
OT  - flow cytometry
OT  - immunomagnetic isolation
EDAT- 2015/04/10 06:00
MHDA- 2016/05/26 06:00
CRDT- 2015/04/10 06:00
PHST- 2014/12/13 00:00 [received]
PHST- 2015/03/04 00:00 [revised]
PHST- 2015/03/31 00:00 [accepted]
PHST- 2015/04/10 06:00 [entrez]
PHST- 2015/04/10 06:00 [pubmed]
PHST- 2016/05/26 06:00 [medline]
AID - 10.1002/cyto.b.21246 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2015 Sep-Oct;88(5):338-47. doi: 10.1002/cyto.b.21246. 
      Epub 2015 Apr 18.