PMID- 25855302
OWN - NLM
STAT- MEDLINE
DCOM- 20160225
LR  - 20181113
IS  - 1432-0991 (Electronic)
IS  - 0343-8651 (Linking)
VI  - 71
IP  - 1
DP  - 2015 Jul
TI  - Co-spinning of Silver Nanoparticles with Nisin Increases the Antimicrobial 
      Spectrum of PDLLA: PEO Nanofibers.
PG  - 24-30
LID - 10.1007/s00284-015-0813-y [doi]
AB  - Silver nanoparticles (AgNPs), synthesized using N,N-dimethylformamide (DMF), were 
      electrospun with nisin in a 50:50 blend of 24 % (w/v) poly(D,L-lactide) (PDLLA) 
      and poly(ethylene oxide) (PEO). Addition of AgNPs decreased the average diameter 
      of the nanofibers [silver nanofibers (SF)] from 588 +/- 191 to 281 +/- 64 nm, or to 
      288 +/- 63 nm when nisin was co-spun with AgNPs. Nanofibers containing AgNO3 (SF) 
      had a beads-on-string structure, whereas nanofibers with AgNPs and nisin [silver 
      plus nisin nanofibers (SNF)], nanofibers with only nisin [nisin nanofibers (NF)], 
      and nanofibers without AgNPs and nisin [control nanofibers] had a uniform 
      structure. The irregular topography was confirmed by atomic force microscopy. No 
      interactions occurred between silver, nisin, PDLLA, and PEO, as confirmed with 
      Fourier transform infrared spectroscopy. Most of the AgNPs (18 +/- 2.8 ppm) and 
      nisin (78.1 +/- 1.2 microg/ml) were released within the first 2 h. SF and SNF inhibited 
      the growth of gram-positive and gram-negative bacteria, whereas NF failed to 
      inhibit gram-negative bacteria. A wound dressing with broad-spectrum 
      antimicrobial activity may be developed by the incorporation of nanofibers 
      containing a combination of AgNPs and nisin.
FAU - Ahire, Jayesh J
AU  - Ahire JJ
AD  - Department of Microbiology, University of Stellenbosch, Matieland, Stellenbosch, 
      7602, South Africa.
FAU - Neveling, Deon P
AU  - Neveling DP
FAU - Dicks, Leon M T
AU  - Dicks LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150409
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Epoxy Compounds)
RN  - 0 (PEO-poly(lactide))
RN  - 0 (Polyesters)
RN  - 1414-45-5 (Nisin)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Anti-Bacterial Agents/*chemistry/*pharmacology
MH  - Epoxy Compounds/*chemistry/*pharmacology
MH  - Gram-Negative Bacteria/drug effects
MH  - Gram-Positive Bacteria/drug effects
MH  - Microscopy, Atomic Force
MH  - Microscopy, Electron, Scanning
MH  - Nanofibers/*chemistry/ultrastructure
MH  - Nisin/*pharmacology
MH  - Polyesters/*chemistry/*pharmacology
MH  - Silver/*pharmacology
EDAT- 2015/04/10 06:00
MHDA- 2016/02/26 06:00
CRDT- 2015/04/10 06:00
PHST- 2014/12/01 00:00 [received]
PHST- 2015/02/26 00:00 [accepted]
PHST- 2015/04/10 06:00 [entrez]
PHST- 2015/04/10 06:00 [pubmed]
PHST- 2016/02/26 06:00 [medline]
AID - 10.1007/s00284-015-0813-y [doi]
PST - ppublish
SO  - Curr Microbiol. 2015 Jul;71(1):24-30. doi: 10.1007/s00284-015-0813-y. Epub 2015 
      Apr 9.