PMID- 25855805
OWN - NLM
STAT- MEDLINE
DCOM- 20150731
LR  - 20181113
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 43
IP  - 9
DP  - 2015 May 19
TI  - Unique C. elegans telomeric overhang structures reveal the evolutionarily 
      conserved properties of telomeric DNA.
PG  - 4733-45
LID - 10.1093/nar/gkv296 [doi]
AB  - There are two basic mechanisms that are associated with the maintenance of the 
      telomere length, which endows cancer cells with unlimited proliferative 
      potential. One mechanism, referred to as alternative lengthening of telomeres 
      (ALT), accounts for approximately 10-15% of all human cancers. Tumours engaged in 
      the ALT pathway are characterised by the presence of the single stranded 
      5'-C-rich telomeric overhang (C-overhang). This recently identified hallmark of 
      ALT cancers distinguishes them from healthy tissues and renders the C-overhang as 
      a clear target for anticancer therapy. We analysed structures of the 5'-C-rich 
      and 3'-G-rich telomeric overhangs from human and Caenorhabditis elegans, the 
      recently established multicellular in vivo model of ALT tumours. We show that the 
      telomeric DNA from C. elegans and humans forms fundamentally different secondary 
      structures. The unique structural characteristics of C. elegans telomeric DNA 
      that are distinct not only from those of humans but also from those of other 
      multicellular eukaryotes allowed us to identify evolutionarily conserved 
      properties of telomeric DNA. Differences in structural organisation of the 
      telomeric DNA between the C. elegans and human impose limitations on the use of 
      the C. elegans as an ALT tumour model.
CI  - (c) The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic 
      Acids Research.
FAU - Skolakova, Petra
AU  - Skolakova P
AD  - Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., 
      Kralovopolska, 135, 612 65 Brno, Czech Republic.
FAU - Foldynova-Trantirkova, Silvie
AU  - Foldynova-Trantirkova S
AD  - Central European Institute of Technology, Masaryk University, Kamenice 735/5, 625 
      00 Brno, Czech Republic Institute of Parasitology, Academy of Sciences of the 
      Czech Republic, Branisovska, 31, 375 05 Ceske Budejovice, Czech Republic.
FAU - Bednarova, Klara
AU  - Bednarova K
AD  - Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., 
      Kralovopolska, 135, 612 65 Brno, Czech Republic Central European Institute of 
      Technology, Masaryk University, Kamenice 735/5, 625 00 Brno, Czech Republic.
FAU - Fiala, Radovan
AU  - Fiala R
AD  - Central European Institute of Technology, Masaryk University, Kamenice 735/5, 625 
      00 Brno, Czech Republic.
FAU - Vorlickova, Michaela
AU  - Vorlickova M
AD  - Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., 
      Kralovopolska, 135, 612 65 Brno, Czech Republic Central European Institute of 
      Technology, Masaryk University, Kamenice 735/5, 625 00 Brno, Czech Republic 
      mifi@ibp.cz.
FAU - Trantirek, Lukas
AU  - Trantirek L
AD  - Central European Institute of Technology, Masaryk University, Kamenice 735/5, 625 
      00 Brno, Czech Republic lukas.trantirek@ceitec.muni.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150408
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/genetics
MH  - DNA/*chemistry
MH  - *Evolution, Molecular
MH  - Humans
MH  - Nucleic Acid Conformation
MH  - Telomere/*chemistry
PMC - PMC4482068
EDAT- 2015/04/10 06:00
MHDA- 2015/08/01 06:00
PMCR- 2015/04/08
CRDT- 2015/04/10 06:00
PHST- 2015/03/25 00:00 [accepted]
PHST- 2015/01/04 00:00 [received]
PHST- 2015/04/10 06:00 [entrez]
PHST- 2015/04/10 06:00 [pubmed]
PHST- 2015/08/01 06:00 [medline]
PHST- 2015/04/08 00:00 [pmc-release]
AID - gkv296 [pii]
AID - 10.1093/nar/gkv296 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2015 May 19;43(9):4733-45. doi: 10.1093/nar/gkv296. Epub 2015 
      Apr 8.