PMID- 25859290
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150410
LR  - 20181113
IS  - 1868-7075 (Print)
IS  - 1868-7083 (Electronic)
IS  - 1868-7075 (Linking)
VI  - 7
IP  - 1
DP  - 2015
TI  - Differences in DNA methylation profile of Th1 and Th2 cytokine genes are 
      associated with tolerance acquisition in children with IgE-mediated cow's milk 
      allergy.
PG  - 38
LID - 10.1186/s13148-015-0070-8 [doi]
LID - 38
AB  - BACKGROUND: Epigenetic changes in DNA methylation could regulate the expression 
      of several allergy-related genes. We investigated whether tolerance acquisition 
      in children with immunoglobulin E (IgE)-mediated cow's milk allergy (CMA) is 
      characterized by a specific DNA methylation profile of Th2 (IL-4, IL-5) and Th1 
      (IL-10, IFN-gamma)-associated cytokine genes. RESULTS: DNA methylation of CpGs in the 
      promoting regions of genes from peripheral blood mononuclear cells and serum 
      level of IL-4, IL-5, IL-10 and INF-gamma were assessed in children with active 
      IgE-mediated CMA (group 1), in children who acquired tolerance to cow's milk 
      proteins (group 2) and in healthy children (group 3). Forty children (24 boys, 
      aged 3 to 18 months) were enrolled: 10 in group 1, 20 in group 2, and 10 in the 
      control group. The DNA methylation profiles clearly separated active CMA patients 
      from healthy controls. We observed an opposite pattern comparing subjects with 
      active IgE-mediated CMA with healthy controls and group 2 children who outgrew 
      CMA. The IL-4 and IL-5 DNA methylation was significantly lower, and IL-10 and 
      INF-gamma DNA methylation was higher in active IgE-mediated CMA patients. Gene 
      promoter DNA methylation rates of all cytokines and respective serum levels were 
      strongly correlated. Formula selection significantly influenced cytokine DNA 
      methylation profiles in group 2. CONCLUSIONS: Tolerance acquisition in children 
      with IgE-mediated CMA is characterized by a distinct Th1 and Th2 cytokine gene 
      DNA methylation pattern. These results suggest that DNA methylation may be a 
      target for CMA prevention and treatment.
FAU - Berni Canani, Roberto
AU  - Berni Canani R
AD  - Department of Translational Medical Science, University of Naples 'Federico II', 
      Via S.Pansini, 5 80131 Naples, Italy ; European Laboratory for the Investigation 
      of Food-Induced Diseases, University of Naples 'Federico II', Via S.Pansini, 5 
      80131 Naples, Italy ; CEINGE-Biotecnologie Avanzate s.c.ar.l, University of 
      Naples 'Federico II', Via S.Pansini, 5 80131 Naples, Italy.
FAU - Paparo, Lorella
AU  - Paparo L
AD  - Department of Translational Medical Science, University of Naples 'Federico II', 
      Via S.Pansini, 5 80131 Naples, Italy.
FAU - Nocerino, Rita
AU  - Nocerino R
AD  - Department of Translational Medical Science, University of Naples 'Federico II', 
      Via S.Pansini, 5 80131 Naples, Italy ; CEINGE-Biotecnologie Avanzate s.c.ar.l, 
      University of Naples 'Federico II', Via S.Pansini, 5 80131 Naples, Italy.
FAU - Cosenza, Linda
AU  - Cosenza L
AD  - Department of Translational Medical Science, University of Naples 'Federico II', 
      Via S.Pansini, 5 80131 Naples, Italy.
FAU - Pezzella, Vincenza
AU  - Pezzella V
AD  - Department of Translational Medical Science, University of Naples 'Federico II', 
      Via S.Pansini, 5 80131 Naples, Italy.
FAU - Di Costanzo, Margherita
AU  - Di Costanzo M
AD  - Department of Translational Medical Science, University of Naples 'Federico II', 
      Via S.Pansini, 5 80131 Naples, Italy.
FAU - Capasso, Mario
AU  - Capasso M
AD  - Department of Molecular Medicine and Medical Biotechnologies, University of 
      Naples Federico I, Via S.Pansini, 5 80131 Naples, Italy ; CEINGE-Biotecnologie 
      Avanzate s.c.ar.l, University of Naples 'Federico II', Via S.Pansini, 5 80131 
      Naples, Italy.
FAU - Del Monaco, Valentina
AU  - Del Monaco V
AD  - Department of Molecular Medicine and Medical Biotechnologies, University of 
      Naples Federico I, Via S.Pansini, 5 80131 Naples, Italy ; CEINGE-Biotecnologie 
      Avanzate s.c.ar.l, University of Naples 'Federico II', Via S.Pansini, 5 80131 
      Naples, Italy.
FAU - D'Argenio, Valeria
AU  - D'Argenio V
AD  - Department of Molecular Medicine and Medical Biotechnologies, University of 
      Naples Federico I, Via S.Pansini, 5 80131 Naples, Italy ; CEINGE-Biotecnologie 
      Avanzate s.c.ar.l, University of Naples 'Federico II', Via S.Pansini, 5 80131 
      Naples, Italy.
FAU - Greco, Luigi
AU  - Greco L
AD  - Department of Translational Medical Science, University of Naples 'Federico II', 
      Via S.Pansini, 5 80131 Naples, Italy ; European Laboratory for the Investigation 
      of Food-Induced Diseases, University of Naples 'Federico II', Via S.Pansini, 5 
      80131 Naples, Italy.
FAU - Salvatore, Francesco
AU  - Salvatore F
AD  - Department of Molecular Medicine and Medical Biotechnologies, University of 
      Naples Federico I, Via S.Pansini, 5 80131 Naples, Italy ; CEINGE-Biotecnologie 
      Avanzate s.c.ar.l, University of Naples 'Federico II', Via S.Pansini, 5 80131 
      Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20150331
PL  - Germany
TA  - Clin Epigenetics
JT  - Clinical epigenetics
JID - 101516977
PMC - PMC4391731
OTO - NOTNLM
OT  - Epigenetics
OT  - Extensively hydrolyzed casein formula
OT  - Food allergy
OT  - Hypoallergenic formulae
OT  - Interferon-gamma
OT  - Interleukin-10
OT  - Interleukin-4
OT  - Interleukin-5
OT  - Lactobacillus rhamnosus GG
EDAT- 2015/04/11 06:00
MHDA- 2015/04/11 06:01
PMCR- 2015/03/31
CRDT- 2015/04/11 06:00
PHST- 2014/12/22 00:00 [received]
PHST- 2015/03/10 00:00 [accepted]
PHST- 2015/04/11 06:00 [entrez]
PHST- 2015/04/11 06:00 [pubmed]
PHST- 2015/04/11 06:01 [medline]
PHST- 2015/03/31 00:00 [pmc-release]
AID - 70 [pii]
AID - 10.1186/s13148-015-0070-8 [doi]
PST - epublish
SO  - Clin Epigenetics. 2015 Mar 31;7(1):38. doi: 10.1186/s13148-015-0070-8. 
      eCollection 2015.