PMID- 25859630
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150411
LR  - 20200929
IS  - 2079-4983 (Print)
IS  - 2079-4983 (Electronic)
IS  - 2079-4983 (Linking)
VI  - 6
IP  - 2
DP  - 2015 Apr 9
TI  - Development of thermosensitive hydrogels of chitosan, sodium and magnesium 
      glycerophosphate for bone regeneration applications.
PG  - 192-203
LID - 10.3390/jfb6020192 [doi]
AB  - Thermosensitive injectable hydrogels based on chitosan neutralized with sodium 
      beta-glycerophosphate (Na-beta-GP) have been studied as biomaterials for drug 
      delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate 
      adhesion and proliferation of bone forming cells. With the aim of improving the 
      suitability of the aforementioned chitosan hydrogels as materials for bone 
      regeneration, Mg was incorporated by partial substitution of Na-beta-GP with 
      magnesium glycerophosphate (Mg-GP). Chitosan/Na-beta-GP and chitosan/Na-beta-GP/Mg-GP 
      hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which 
      induces hydrogel mineralization. Hydrogels were characterized physicochemically 
      with respect to mineralizability and gelation kinetics, and biologically with 
      respect to cytocompatibility and cell adhesion. Substitution of Na-beta-GP with 
      Mg-GP did not negatively influence mineralizability. Cell biological testing 
      showed that both chitosan/Na-beta-GP and chitosan/Na-beta-GP/Mg-GP hydrogels were 
      cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-beta-GP/Mg-GP 
      hydrogels can be used as an alternative to chitosan/Na-beta-GP hydrogels for bone 
      regeneration applications. However the incorporation of Mg in the hydrogels 
      during hydrogel formation did not bring any appreciable physicochemical or 
      biological benefit.
FAU - Liskova, Jana
AU  - Liskova J
AD  - Department of Biomaterials and Tissue Engineering, Institute of Physiology of the 
      Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic. 
      janefox@biolog.cz.
FAU - Bacakova, Lucie
AU  - Bacakova L
AD  - Department of Biomaterials and Tissue Engineering, Institute of Physiology of the 
      Czech Academy of Sciences, Videnska 1083, Prague, Czech Republic. 
      lucy@biomed.cas.cz.
FAU - Skwarczynska, Agata L
AU  - Skwarczynska AL
AD  - Faculty of Civil Environmental Engineering and Architecture, Rzeszow University 
      of Technology, ul. Poznanska 2, Rzeszow 35-084, Poland. askwarczynska1@wp.pl.
FAU - Musial, Olga
AU  - Musial O
AD  - Polymer Chemistry and Biomaterials (PBM) Group, Department of Organic Chemistry, 
      Ghent University, Krijgslaan 281 S4, Gent 9000, Belgium. olga.musial@op.pl.
FAU - Bliznuk, Vitaliy
AU  - Bliznuk V
AD  - Department Material Science & Engineering, Ghent University, Technologiepark 903, 
      Zwijnaarde 9052, Belgium. vitaliy.bliznuk@ugent.be.
FAU - De Schamphelaere, Karel
AU  - De Schamphelaere K
AD  - Laboratory for Environmental and Aquatic Ecology, Environmental Toxicology Unit 
      (GhEnToxLab), Faculty of Bioscience Engineering, Ghent University, Jozef 
      Plateaustraat 22, Gent 9000, Belgium. Karel.Deschamphelaere@ugent.be.
FAU - Modrzejewska, Zofia
AU  - Modrzejewska Z
AD  - Department of Environmental Systems Engineering, Faculty of Process and 
      Environmental Engineering, Technical University of Lodz, ul. Wolczanska 213, Lodz 
      90-924, Poland. zofia.modrzejewska@p.lodz.pl.
FAU - Douglas, Timothy E L
AU  - Douglas TE
AD  - Polymer Chemistry and Biomaterials (PBM) Group, Department of Organic Chemistry, 
      Ghent University, Krijgslaan 281 S4, Gent 9000, Belgium. 
      timothy.douglas@ugent.be.
LA  - eng
PT  - Journal Article
DEP - 20150409
PL  - Switzerland
TA  - J Funct Biomater
JT  - Journal of functional biomaterials
JID - 101570734
PMC - PMC4493507
EDAT- 2015/04/11 06:00
MHDA- 2015/04/11 06:01
PMCR- 2015/06/01
CRDT- 2015/04/11 06:00
PHST- 2014/12/24 00:00 [received]
PHST- 2015/03/31 00:00 [revised]
PHST- 2015/04/01 00:00 [accepted]
PHST- 2015/04/11 06:00 [entrez]
PHST- 2015/04/11 06:00 [pubmed]
PHST- 2015/04/11 06:01 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - jfb6020192 [pii]
AID - jfb-06-00192 [pii]
AID - 10.3390/jfb6020192 [doi]
PST - epublish
SO  - J Funct Biomater. 2015 Apr 9;6(2):192-203. doi: 10.3390/jfb6020192.