PMID- 25861279
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150411
LR  - 20200929
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2015
DP  - 2015
TI  - The Chondrogenic Induction Potential for Bone Marrow-Derived Stem Cells between 
      Autologous Platelet-Rich Plasma and Common Chondrogenic Induction Agents: A 
      Preliminary Comparative Study.
PG  - 589124
LID - 10.1155/2015/589124 [doi]
LID - 589124
AB  - The interests in platelet-rich plasma (PRP) and their application in stem cell 
      therapy have contributed to a better understanding of the basic biology of the 
      prochondrogenesis effect on bone marrow-derived stem cells (BMSCs). We aimed at 
      comparing the effect of autologous PRP with common chondrogenic induction agents 
      (CCIAs) on the chondrogenic differentiation of BMSCs. Rabbit BMSCs were isolated 
      and characterized by flow cytometry and differentiated towards adipocytes and 
      osteoblasts. The chondrogenic response of BMSCs to autologous PRP and CCIAs which 
      included transforming growth factor-beta1 (TGF-beta1), dexamethasone (DEX), and vitamin 
      C (Vc) was examined by cell pellet culture. The isolated BMSCs after two passages 
      highly expressed CD29 and CD44 but minimally expressed CD45. The osteogenic and 
      adipogenic differentiation potentials of the isolated BMSCs were also confirmed. 
      Compared with common CCIAs, autologous PRP significantly upregulated the 
      chondrogenic related gene expression, including Col-2, AGC, and Sox-9. Osteogenic 
      related gene expression, including Col-1 and OCN, was not of statistical 
      significance between these two groups. Thus, our data shows that, compared with 
      common chondrogenic induction agents, autologous PRP can be more effective in 
      promoting the chondrogenesis of BMSCs.
FAU - Wang, Shan-Zheng
AU  - Wang SZ
AD  - Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast 
      University, 87 Ding Jia Qiao Road, Nanjing, Jiangsu 210009, China.
FAU - Chang, Qing
AU  - Chang Q
AD  - Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast 
      University, 87 Ding Jia Qiao Road, Nanjing, Jiangsu 210009, China.
FAU - Kong, Xiang-Fei
AU  - Kong XF
AD  - Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast 
      University, 87 Ding Jia Qiao Road, Nanjing, Jiangsu 210009, China.
FAU - Wang, Chen
AU  - Wang C
AD  - Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast 
      University, 87 Ding Jia Qiao Road, Nanjing, Jiangsu 210009, China ; Surgical 
      Research Center, Medical School of Southeast University, 87 Ding Jia Qiao Road, 
      Nanjing, Jiangsu 210009, China.
LA  - eng
PT  - Journal Article
DEP - 20150316
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC4378611
EDAT- 2015/04/11 06:00
MHDA- 2015/04/11 06:01
PMCR- 2015/03/16
CRDT- 2015/04/11 06:00
PHST- 2014/10/24 00:00 [received]
PHST- 2014/12/28 00:00 [revised]
PHST- 2015/01/05 00:00 [accepted]
PHST- 2015/04/11 06:00 [entrez]
PHST- 2015/04/11 06:00 [pubmed]
PHST- 2015/04/11 06:01 [medline]
PHST- 2015/03/16 00:00 [pmc-release]
AID - 10.1155/2015/589124 [doi]
PST - ppublish
SO  - Stem Cells Int. 2015;2015:589124. doi: 10.1155/2015/589124. Epub 2015 Mar 16.